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首页> 外文期刊>Journal of Toxicology and Environmental Health, Part A: Current Issues >Detection of Quinone Oxidoreductase 1 (NQO1) Single-Nucleotide Polymorphisms (SNP) Related to Benzenemetabolism in Immortalized B Lymphocytes From A Chinese Han Population
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Detection of Quinone Oxidoreductase 1 (NQO1) Single-Nucleotide Polymorphisms (SNP) Related to Benzenemetabolism in Immortalized B Lymphocytes From A Chinese Han Population

机译：中国汉族人群永生化B淋巴细胞中与苯并代谢相关的醌氧化还原酶1（NQO1）单核苷酸多态性（SNP）的检测。

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摘要

Single-nucleotide polymorphisms (SNP) in genes coding metabolizing enzymes modulate gene functions and cellular toxicity in response to chemicals. Quinone oxidoreductase 1 (NQO1) is an important detoxification enzyme involved in the catabolism of 1,4-benzoquinone (1,4-BQ), a benzene metabolite believed to be associated with bone-marrow toxicity and leukemia. Gene function was evaluated in immortalized human B lymphocytes derived from a Chinese Han population with independent genotypes at 2 NQO1 SNP sites. 1,4-Benzoquinone was incubated with these immortalized lymphocytes of differing genotypes. Among the genotypes of 2 SNP examined, cell lines with rs1800566CC showed a higher NQO1 enzymic activity after a 48 h of treatment with 10 μM 1,4-BQ, and a lower comet rate compared with cells of CT/TT genotypes. Data suggested that NQO1 rs1800566 might serve as a functional genetic marker for benzene toxicity in the Chinese Han population. The immortalized B lymphocytes derived from different populations might thus be used as a biomarker to detect functional genetic markers related to exposure to environmental chemicals.
机译：编码代谢酶的基因中的单核苷酸多态性（SNP）调节基因功能和细胞毒性以响应化学物质。醌氧化还原酶1（NQO1）是一种重要的解毒酶，涉及1,4-苯醌（1,4-BQ）的分解代谢，该苯代谢物被认为与骨髓毒性和白血病有关。在来自中国汉族的永生化人类B淋巴细胞中评估了基因功能，该汉族具有2个NQO1 SNP位点的独立基因型。 1,4-苯醌与这些永生化的不同基因型淋巴细胞一起孵育。在基因型的2个SNP中，rs1800566CC细胞系显示更高的NQO1酶活性后处理10 µM 1,4-BQ 48小时，与CT / TT基因型的细胞相比彗星发生率更低。数据表明，NQO1 rs1800566可能是中国汉族人群苯毒性的功能遗传标记。因此，来自不同种群的永生化B淋巴细胞可以用作生物标志物，以检测与环境化学物暴露相关的功能性遗传标志物。

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