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首页> 外文期刊>Journal of water supply >Possible health impacts due to animal and human fecal pollution in water intended for drinking water supply of Rio de Janeiro, Brazil
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Possible health impacts due to animal and human fecal pollution in water intended for drinking water supply of Rio de Janeiro, Brazil

机译：巴西里约热内卢的饮用水供应中的动物和人类粪便污染可能对健康造成影响

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Fecal matter is considered as one of the worst pollutants in waterbodies due to the potential spread of waterborne diseases. This study aimed to determine the host-specific fecal contamination in two Brazilian watersheds and to predict the possible impacts on human health. Fecal sources were enumerated using host-specific genetic markers to swine (16S rRNA), human and bovine (archaeal nifH), and equine (archaeal mcrA). A single cycling condition was established for four markers aiming to decrease the analysis time. Fifteen samples from Sao Joao watershed (75%) and 25 from Guandu (62.5%) presenting Escherichia coli enumeration in compliance with Brazilian guidelines (<1,000 MPN/100 mL) showed the human marker. Furthermore, the bovine, swine, and equine markers were present in 92% (59/64), 89% (57/64), and 81% (52/64) of the water samples, respectively. The molecular markers proposed for qPCR in our study were sensitivity and specific enough to detect host-specific fecal pollution in all samples regardless of E. coli levels reaffirming the low correlation among them and supporting their use in water quality monitoring programs. To our knowledge, this is the first study using this approach for quantification of nifH, mcrA, and rrs gene-associated human and animal fecal pollution in waters intended for drinking water supply in Brazil.
机译：由于水传播疾病的潜在传播，粪便被认为是水体中最严重的污染物之一。这项研究旨在确定两个巴西流域中特定于宿主的粪便污染，并预测对人类健康的可能影响。使用宿主特异性遗传标记对猪（16S rRNA），人和牛（古细菌nifH）和马（古细菌mcrA）进行粪便来源计数。针对四个标记物建立了单个循环条件，旨在减少分析时间。根据巴西指南（<1,000 MPN / 100 mL），来自圣若昂（Sao Joao）流域的15个样本（占75％）和来自冠渡（25.5％）的25个样本进行了大肠杆菌计数，显示出大肠杆菌。此外，牛，猪和马标记分别存在于92％（59/64），89％（57/64）和81％（52/64）的水样本中。在我们的研究中建议用于qPCR的分子标记物具有敏感性和特异性，足以检测所有样品中的宿主特异性粪便污染，而与大肠杆菌水平无关，从而重申了它们之间的低相关性并支持其在水质监测程序中的使用。据我们所知，这是巴西首次使用这种方法对与饮用水相关的水中与nifH，mcrA和rrs基因相关的人类和动物粪便污染进行定量研究。

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