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首页> 外文期刊>Journal of Wood Chemistry and Technology >A One-Step Method for the Simultaneous Determination of Five Wood Monosaccharides and the Corresponding Aldonic Acids in Fermentation Broth Using High-Performance Anion-Exchange Chromatography Coupled with a Pulsed Amperometric Detector
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A One-Step Method for the Simultaneous Determination of Five Wood Monosaccharides and the Corresponding Aldonic Acids in Fermentation Broth Using High-Performance Anion-Exchange Chromatography Coupled with a Pulsed Amperometric Detector

机译：高效阴离子交换色谱-脉冲安培检测器同时测定发酵液中五种木材单糖和相应的醛酸的一步法

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摘要

Interest in biomass biorefineries and especially their aldonic acid products has been growing in recent years. However, the analytical methods for aldonic acids have only rarely been explored. In this study, a simple one-step method was developed using high-performance anion-exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD). With this method, five wood monosaccharides and their corresponding aldonic acids, including arabinose, galactose, glucose, xylose, mannose, arabonic acid, galactonic acid, gluconic acid, xylonic acid, and mannonic acid, were simultaneously separated and quantitatively determined. The separation was performed on a CarboPac™ PA-10 column (250 mm × 2 mm) with a gradient elution using NaOH solution as the mobile phase. The calibration curves showed good linearity (R~2 ≥ 0.9993) for the five monosaccharides and the corresponding aldonic acids in the range of 0.1 to 10.0 mg/L. The spiked recoveries of the 10 components ranged from 93.92% to 104.15%, with relative standard deviations (RSDs) (n = 3) of 0.49% to 3.95%. The established method was applied successfully to determine the quantitative variation of monosaccharide and aldonic acid contents in products from a biomass biorefinery.
机译：近年来，对生物质生物精炼厂，特别是其醛糖酸产品的兴趣不断增长。但是，很少探索醛糖酸的分析方法。在这项研究中，使用高性能阴离子交换色谱结合脉冲安培检测（HPAEC-PAD）开发了一种简单的一步方法。通过这种方法，同时分离并定量测定了五种木材单糖及其相应的醛糖酸，包括阿拉伯糖，半乳糖，葡萄糖，木糖，甘露糖，阿拉伯糖酸，半乳糖酸，葡糖酸，木糖酸和甘露酸。分离在CarboPac™PA-10色谱柱（250 mm×2 mm）上进行，使用NaOH溶液作为流动相进行梯度洗脱。五个单糖和相应的醛糖酸在0.1至10.0 mg / L范围内的校准曲线均显示出良好的线性（R〜2≥0.9993）。 10种组分的加标回收率范围为93.92％至104.15％，相对标准偏差（RSD）（n = 3）为0.49％至3.95％。所建立的方法已成功应用于测定生物质生物精炼厂产品中单糖和醛糖酸含量的定量变化。

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  • 来源
    《Journal of Wood Chemistry and Technology》 |2014年第1期|67-76|共10页
  • 作者

    XING WANG; YONG XU; ZHINA LIAN; QIANG YONG; SHIYUAN YU;

  • 作者单位

    College of Chemical Engineering, Nanjing Forestry University, Nanjing, Jiangsu Province, China Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing, China;

    College of Chemical Engineering, Nanjing Forestry University, Nanjing, Jiangsu Province, China Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing, China Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals, Nanjing, China;

    College of Chemical Engineering, Nanjing Forestry University, Nanjing, Jiangsu Province, China Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing, China;

    College of Chemical Engineering, Nanjing Forestry University, Nanjing, Jiangsu Province, China Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing, China;

    College of Chemical Engineering, Nanjing Forestry University, Nanjing, Jiangsu Province, China Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing, China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Monosaccharides; aldonic acids; simultaneous separation and determination; HPAEC-PAD;

    机译：单糖醛糖酸同时分离和测定;HPAEC-PAD;

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