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How microcystin-degrading bacteria express microcystin degradation activity

机译：微囊藻毒素降解细菌如何表达微囊藻毒素降解活性

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摘要

Alkali tolerance and the mechanism of microcystin (MC) degradation were investigated in the MC-degrading bacterial species, Sphingopyxis sp. C-l, to better understand the increased MC degradation under the alkaline conditions that arise during the disappearance of water blooms. MC-degrading bacteria harbour mlrA, mlrB and mlrC that encode MC-degrading enzymes. Sphingopyxis sp. C-l also possesses these genes, as well as the mlrD gene that has been assumed to encode MC and its degradation transporter. This study demonstrated that MC degradation activity was promoted by the intermittent addition of microcystin-LR (MCLR) to cultures of strain C-l. That the expression of mlrA, mlrB and mlrC is induced by MCLR also was indicated, whereas that of mlrA and mlrB is induced by the MCLR degradation products linear MC, H-Adda-Glu-Mdha-Ala-OH (tetra peptide) and 2S, 3S, 8S, 9S-3-amino-9-methoxy-2, 6, 8-trimethyl-10-phenyldeca-4E, 6E-dienoic acid (Adda). Adda played a key role in the induction of mlrA and mlrB gene expression, and the cyclic structure of MCLR was closely associated with the induction of mlrC gene expression. It is suggested, therefore, that Adda is an essential part of a signalling molecule involved in cell-to-cell communication. Finally, the MC-degrading bacteria responded to MCLR and its degradation products by degrading the MlrA, MlrB and MlrC enzymes through a sequential chain reaction for the expression of each.
机译：研究了碱的耐受性和微囊藻毒素（MC）降解机制在降解MC的细菌物种Sphingopyxis sp。中的作用。 C-1，以更好地了解在水华消失过程中出现的碱性条件下MC降解的增加。降解MC的细菌带有编码MC降解酶的mlrA，mlrB和mlrC。鞘氨醇单胞菌C-1还拥有这些基因，以及已经假定编码MC及其降解转运蛋白的mlrD基因。该研究表明，通过向菌株C-1的培养物中间歇添加微囊藻毒素-LR（MCLR），促进了MC降解活性。还表明了MCLR诱导mlrA，mlrB和mlrC的表达，而MCLR降解产物线性MC，H-Adda-Glu-Mdha-Ala-OH（四肽）和2S诱导了mlrA和mlrB的表达。 ，3S，8S，9S-3-氨基-9-甲氧基-2、6、8-三甲基-10-苯基癸4E，6E-二烯酸（Adda）。 Adda在诱导mlrA和mlrB基因表达中起关键作用，而MCLR的环状结构与诱导mlrC基因表达密切相关。因此，建议Adda是参与细胞间通信的信号分子的重要组成部分。最后，降解MC的细菌通过顺序表达的顺序反应降解MlrA，MlrB和MlrC酶，从而对MCLR及其降解产物产生反应。

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  • 来源
    《Lakes & Reservoirs》 |2011年第3期|p.169-178|共10页
  • 作者

    Kazuya Shimizu; Hideaki Maseda; Kunihiro Okano; Tomoaki Itayama; Yukio Kawauchi; Rongzhi Chen; Motoo Utsumi; Zhenya Zhang; Norio Sugiura;

  • 作者单位

    Department of Life Sciences, Toyo University, Izumino Itakura Ora-gun Gunma;

    Department of Biological Science & Technology, Institute of Technology of Science, The University of Tokushima Graduate School, Tokushima 770-8506, Japan;

    Graduate School of Bioresource Science Akita Prefectural University, Kaidobata-Nishi, Nakano Shimoshinjo, Akita City, Akita;

    Research Institute for Human and Nature, Motoyama, Kamigamo, Kita-ku, Kyoto;

    Mitsubishi Chemical Analytech Co. Ltd., Enzo Chigasaki, Kanagawa;

    Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai, Tsukuba, Ibaraki, Japan;

    Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai, Tsukuba, Ibaraki, Japan;

    Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai, Tsukuba, Ibaraki, Japan;

    Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai, Tsukuba, Ibaraki, Japan;

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  • 关键词

    alkali-tolerant microorganisms; cyanobacteria; public health; reservoir management; water bloom;

    机译：耐碱微生物蓝细菌公共卫生;水库管理;水华;

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