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Total bacterial populations in three lentic water bodies of the Colombian Andes using the epifluorescence technique

机译:使用落射荧光技术的哥伦比亚安第斯山脉的三个小透镜水体中的细菌总数

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摘要

The epifluorescence technique was used to quantify the total bacterial population in two dams (Neusa and Prado) and in a natural lagoon (FÚquene), which have different geographical locations and physicochemical characteristics. Bacterial abundance was similar in the three water-bodies (8.4 × 108 cells L–1), but the density of plateable heterotrophs was always lower (1.4 × 106 CFU L–1) when obtained by the plate count method. Results obtained during validation of the epifluorescence technique show: (i) that there are no statistically significant differences between counts of samples fixed in the field and unfixed samples; (ii) that the median count of samples fixed and observed periodically over a period of 8 months was similar; (iii) that plate counts underestimate the population; and (iv) that epifluorescence counts were similar in the three water-bodies studied, despite their different ecological conditions.
机译:落射荧光技术用于量化两个大坝(Neusa和Prado)和天然泻湖(Fquene)中的细菌总数,这两个大坝的地理位置和理化特性不同。在三个水体中细菌丰度相似(8.4×108细胞L-1),但通过平板计数法获得的可电镀异养菌密度始终较低(1.4×106 CFU L-1)。在落射荧光技术验证过程中获得的结果表明:(i)在现场固定的样品数量与未固定的样品数量之间没有统计学上的显着差异; (ii)在8个月的时间里定期固定和观察的样本中位数计数是相似的; (iii)车牌数量低估了人口; (iv)尽管所研究的三个水体的生态条件不同,但它们的落荧光数却相似。

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  • 来源
    《Lakes & Reservoirs》 |2001年第2期|169-174|共6页
  • 作者

    Amparo Canosa; Gabriel Pinilla;

  • 作者单位

    Microbiology Laboratory and Scientific Research and Environmental Studies Center Universidad de BogotÁ Jorge Tadeo Lozano Carrera 4 no. 22–61 BogotÁ Colombia;

    Microbiology Laboratory and Scientific Research and Environmental Studies Center Universidad de BogotÁ Jorge Tadeo Lozano Carrera 4 no. 22–61 BogotÁ Colombia;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Andean region; bacterioplankton; epifluorescence; plate counts;

    机译:安第斯地区;浮游细菌;荧光;平板计数;

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