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首页> 外文期刊>Lipids >In Vitro Simultaneous Transfer of Lipids to HDL in Coronary Artery Disease and in Statin Treatment
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In Vitro Simultaneous Transfer of Lipids to HDL in Coronary Artery Disease and in Statin Treatment

机译：在冠状动脉疾病和他汀类药物治疗中脂质同时向HDL的同时转移

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摘要

The exchange of lipids with cells and other lipoproteins is a crucial process in HDL metabolism and for HDL antiatherogenic function. Here, we tested a practical method to quantify the simultaneous transfer to HDL of phospholipids, free-cholesterol, esterified cholesterol and triacylglycerols and to verify the lipid transfer in patients with coronary artery disease (CAD) or undergoing statin treatment. Twenty-eight control subjects without CAD, 27 with CAD and 25 CAD patients under simvastatin treatment were studied. Plasma samples were incubated with a donor nanoemulsion prepared by ultrasonication of the constituent lipids and labeled with radioactive lipids; % lipids transferred to HDL were quantified in the HDL-containing supernatant after chemical precipitation of non-HDL fractions and the nanoemulsion. The assay was precise and reproducible. Increase of temperature (4–37 °C), of incubation period (5 min to 2 h), of HDL-cholesterol concentration (33–244 mg/dL) and of mass of nanoemulsion lipids (0.075–0.3 mg/μL) resulted in increased lipid transfer from the nanoemulsion to HDL. In contrast, increasing pH (6.5–8.5) and albumin concentration (3.5–7.0 g/dL) did not affect lipid transfer. There was no difference between CAD and control non-CAD with regard to the lipid transfer, but statin treatment reduced the transfer to HDL of all four lipids. The test herein described is a valid and practical tool for exploring an important aspect of HDL metabolism. Keywords High-density lipoprotein - Nanoemulsions - Coronary artery disease - Statins - Nanoparticles - Transfer proteins - Cholesterol - Triacylglycerols - Cholesteryl ester transfer protein (CETP) - Phospholipid transfer protein (PLTP)
机译：脂质与细胞和其他脂蛋白的交换是HDL代谢和HDL抗动脉粥样硬化功能的关键过程。在这里，我们测试了一种实用的方法来量化磷脂，游离胆固醇，酯化胆固醇和三酰甘油同时向HDL的转移，并验证患有冠心病（CAD）或接受他汀类药物治疗的患者的脂质转移。研究人员对28名无CAD的对照受试者，27名有CAD的受试者和25名接受辛伐他汀治疗的CAD患者进行了研究。将血浆样品与通过超声成分脂质制备的供体纳米乳一起孵育，并用放射性脂质标记。在非HDL馏分和纳米乳剂的化学沉淀之后，在含HDL的上清液中定量转移至HDL的脂质％。该测定法是精确且可重复的。结果导致温度升高（4–37°C），潜伏期（5分钟至2小时），HDL-胆固醇浓度（33–244 mg / dL）和纳米乳液脂质的质量（0.075–0.3 mg /μL）增加脂质从纳米乳液向HDL的转移增加。相反，增加pH（6.5-8.5）和白蛋白浓度（3.5-7.0 g / dL）不会影响脂质转移。就脂质转移而言，CAD与非对照非CAD之间没有差异，但是他汀类药物治疗减少了所有四种脂质向HDL的转移。本文所述的测试是探索HDL代谢重要方面的有效且实用的工具。关键词高密度脂蛋白-纳米乳液-冠状动脉疾病-他汀类药物-纳米颗粒-转移蛋白-胆固醇-三酰甘油-胆固醇酯转移蛋白（CETP）-磷脂转移蛋白（PLTP）

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