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首页> 外文期刊>Lipids >Determination of Tertiary-butylhydroquinone and Its Metabolites in Rat Serum by Liquid Chromatography–Ion Trap Mass Spectrometry
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Determination of Tertiary-butylhydroquinone and Its Metabolites in Rat Serum by Liquid Chromatography–Ion Trap Mass Spectrometry

机译：液相色谱-离子阱质谱法测定大鼠血清中叔丁基对苯二酚及其代谢物

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摘要

A new method applying sensitive and selective liquid chromatography coupled with mass spectrometry (LC/MS/MS) for analyzing tertiary-butylhydroquinone (TBHQ) and its metabolites in rat serum was validated. Using an extracted ion chromatogram (EIC) of m/z 149, free TBHQ was observed in rat serum after dosing TBHQ at 350 mg/kg to male and female Sprague–Dawley (SD) rats. Four major metabolites of TBHQ were identified—a TBHQ-sulfate, two TBHQ-sulfate-derived substances and a TBHQ-glucuronide through MSn spectra. Besides its simplicity, the sample treatment allows one to obtain a very good recovery of analysts, namely around 95%. This result suggests that the method described here is useful for the analysis of TBHQ and its metabolites in rat serum. Moreover, the metabolism of TBHQ was investigated using the method. After oral administration, TBHQ appear to be more completely absorbed and bio-transformed by males than females, which may result in higher acute oral toxicity of TBHQ for males than females.
机译：验证了一种应用灵敏和选择性液相色谱结合质谱（LC / MS / MS）分析大鼠血清中叔丁基对苯二酚（TBHQ）及其代谢物的新方法。使用m / z 149的提取离子色谱图（EIC），在雄性和雌性Sprague-Dawley（SD）大鼠服用TBHQ 350 mg / kg后，在大鼠血清中观察到游离TBHQ。通过MS n 光谱鉴定出了TBHQ的四种主要代谢产物-硫酸TBHQ，两种源自TBHQ的物质和TBHQ-葡糖醛酸。除了简单之外，样本处理还可以使分析人员获得很好的回收率，即大约95％。该结果表明，此处描述的方法可用于分析大鼠血清中的TBHQ及其代谢物。此外，使用该方法研究了TBHQ的代谢。口服后，男性比女性更容易吸收和生物转化TBHQ，这可能导致男性对TBHQ的急性口服毒性高于女性。

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