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首页> 外文期刊>Marine biology >Development of a sensitive detection method to survey pelagic biodiversity using eDNA and quantitative PCR: a case study of devil ray at seamounts
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Development of a sensitive detection method to survey pelagic biodiversity using eDNA and quantitative PCR: a case study of devil ray at seamounts

机译：开发一种灵敏的检测方法，利用eDNA和定量PCR来调查中上层生物多样性：以海山上的魔鬼鱼为例

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Environmental DNA (eDNA) is increasingly being used in aquatic environments for monitoring species, particularly those that are of conservation concern and/or are difficult to visually observe. Quantitative PCR (qPCR) has been employed to detect low abundance species occurring in environmental water samples. However, the qPCR technique has principally been applied to freshwater habitats, with less application to pelagic marine environments. We developed a species-specific eDNA assay for the Chilean devil ray, Mobula tarapacana, to assess the capability of using eDNA to detect transient pelagic marine animals. For this pilot study, seawater samples taken at seamounts around the Azores (NE Atlantic) were tested to determine the suitability of this approach for detecting the target species. Samples were taken at sites where M. tarapacana has been previously observed, in addition to sites where its presence is not known. eDNA detection was compared to observations carried out on the same day as water sampling. The qPCR assay successfully detected M. tarapacana at four of five seamount sampling opportunities where the species was observed, and there is a statistically significant relationship between genetic and visual detection. Target DNA was found at one location between seamounts in the absence of visual observation. Our results highlight the importance of physical environmental factors in relation to sampling eDNA in the ocean, such as currents and eDNA dispersal ability. This method has been shown to be sensitive for detection of M. tarapacana DNA in seawater and, therefore, in the identification of important seamounts requiring conservation.
机译：环境DNA（eDNA）越来越多地用于水生环境中，以监测物种，特别是那些具有保护意义和/或难以目视观察的物种。定量PCR（qPCR）已用于检测环境水样品中发生的低丰度物质。但是，qPCR技术主要应用于淡水生境，而对远洋海洋环境的应用则较少。我们开发了一种针对智利魔鬼Mobula tarapacana的物种特异性eDNA分析，以评估使用eDNA检测瞬态上层海洋动物的能力。对于该初步研究，测试了在亚速尔群岛（北大西洋）附近海山采集的海水样品，以确定这种方法是否适合检测目标物种。除了不知道其存在的地点外，还从以前曾观察到塔拉帕卡纳分枝的地点取样。将eDNA检测与在水采样当天进行的观察结果进行了比较。 qPCR测定法在观察到该物种的五个海山采样机会中的四个机会中成功检测到了塔拉帕卡纳分枝杆菌，并且在遗传和视觉检测之间存在统计学上的显着关系。在没有目视观察的情况下，在海山之间的一个位置发现了目标DNA。我们的结果强调了与采样海洋中eDNA有关的物理环境因素的重要性，例如洋流和eDNA的扩散能力。已经表明，该方法对于检测海水中的塔拉帕卡纳分枝杆菌DNA敏感，因此，在识别需要保护的重要海山中也很灵敏。

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    《Marine biology》 |2017年第5期|112.1-112.9|共9页
  • 作者

    Gargan Laura M.; Morato Telmo; Pham Christopher K.; Finarelli John A.; Carlsson Jeanette E. L.; Carlsson Jens;

  • 作者单位

    Univ Coll Dublin, Area Res Grp 52, Dublin 4, Ireland|Univ Coll Dublin, Earth Inst, Sch Biol & Environm Sci, Dublin 4, Ireland;

    Univ Acores, Inst Mar, P-9901862 Horta, Portugal|Univ Acores, Marine & Environm Sci Ctr, P-9901862 Horta, Portugal;

    Univ Acores, Inst Mar, P-9901862 Horta, Portugal|Univ Acores, Marine & Environm Sci Ctr, P-9901862 Horta, Portugal;

    Univ Coll Dublin, Earth Inst, Sch Biol & Environm Sci, Dublin 4, Ireland;

    Univ Coll Dublin, Area Res Grp 52, Dublin 4, Ireland|Univ Coll Dublin, Earth Inst, Sch Biol & Environm Sci, Dublin 4, Ireland;

    Univ Coll Dublin, Area Res Grp 52, Dublin 4, Ireland|Univ Coll Dublin, Earth Inst, Sch Biol & Environm Sci, Dublin 4, Ireland;

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