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首页> 外文期刊>Marine biology >When warming hits harder: survival, cellular stress and thermal limits of Sparus aurata larvae under global change
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When warming hits harder: survival, cellular stress and thermal limits of Sparus aurata larvae under global change

机译：当变暖受到的打击更大时：全球变化下Sparus aurata幼虫的生存，细胞应激和温度极限

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Understanding physiological and molecular compensation mechanisms that shape thermotolerance is crucial for estimating the effects of ocean warming on fish stocks, especially during early life stages, whose tolerance determines recruitment success and population viability. The aims of this study were to assess the sensitivity of fish larvae toward ocean warming and heat wave events in the commercial species, Sparus aurata, whose habitat is likely to be affected by rising water temperatures. We (1) estimated its critical thermal maximum (CTmax) and relative mortality upon warming, (2) quantified stress biomarkers: heat shock protein 70 kDa, total ubiquitin, antioxidant enzymes (superoxide dismutase, catalase, glutathione-S-transferase), lipid peroxidation and protein carbonylation, and (3) analyzed histopathological changes as a result of thermal stress. Larvae showed increasing levels of lethargy with increasing temperature, attaining a cumulative CTmax value of 30 degrees C. Relative mortality increased upon warming, reaching 80 % at 30 degrees C. Oxidative damage was higher at moderate temperatures and decreased at 24 degrees C probably due to a significant increase in superoxide dismutase's (SODs) activity. Hsp70 chaperone levels also increased at 26 degrees C, but unfolding persisted at higher temperatures as shown by the increase in total ubiquitin at 26 and 28 degrees C, indicating protein damage. Skeletal muscle showed disorganization of muscle fibers from 24 degrees C onwards. Overall, protein denaturation seems to be the major cause of larval mortality, potentially compromising recruitment's success from 22 degrees C onwards, since larvae migrate into nursery grounds by spring and summer (i.e., high temperatures), thus hindering the viability of local fish stocks. These data demonstrate that the biochemical homeostasis of fish can be disturbed within an ecologically realistic thermal range and emphasize the risks of rising global temperatures for larval fishes.
机译：了解影响耐热性的生理和分子补偿机制对于估计海洋变暖对鱼类种群的影响至关重要，尤其是在生命的早期阶段，其耐受性决定了募集成功和种群生存力。这项研究的目的是评估商业化物种Sparus aurata中鱼幼虫对海洋变暖和热浪事件的敏感性，其栖息地可能会受到水温升高的影响。我们（1）估算其变暖时的临界最大热值（CTmax）和相对死亡率，（2）量化的应激生物标记物：热休克蛋白70 kDa，总泛素，抗氧化酶（超氧化物歧化酶，过氧化氢酶，谷胱甘肽S-转移酶），脂质（3）分析了热应激导致的组织病理学变化。幼虫的嗜睡程度随温度的升高而增加，累积的CTmax值达到30摄氏度。相对死亡率随温度升高而增加，在30摄氏度时达到80％。在中等温度下，氧化损伤较高，而在24摄氏度时，氧化损伤可能是由于超氧化物歧化酶（SOD）活性的显着提高。 Hsp70伴侣蛋白的水平在26摄氏度时也有所增加，但在较高的温度下仍持续存在，如26和28摄氏度时总泛素的增加所表明的，表明蛋白质受到了破坏。从24摄氏度开始，骨骼肌显示出肌肉纤维的混乱。总体而言，蛋白质变性似乎是造成幼虫死亡的主要原因，因为幼虫在春季和夏季（即高温之前）迁移到育苗场中，因此从22摄氏度开始可能会危害招募工作的成功，从而阻碍了当地鱼类种群的生存。这些数据表明，在生态现实的热范围内，鱼类的生化稳态可能受到干扰，并强调了幼体鱼类全球温度升高的风险。

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  • 来源
    《Marine biology》 |2016年第4期|91.1-91.14|共14页
  • 作者

    Madeira Diana; Costa Pedro M.; Vinagre Catarina; Diniz Mario S.;

  • 作者单位

    Univ Nova Lisboa, UCIBIO REQUIMTE, Fac Ciencias & Tecnol, P-2829516 Caparica, Portugal;

    Karolinska Inst, Inst Environm Med IMM, Unit Mol Toxicol, Nobels Vag 13, S-17177 Stockholm, Sweden;

    Univ Nova Lisboa, UCIBIO REQUIMTE, Fac Ciencias & Tecnol, P-2829516 Caparica, Portugal;

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