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首页> 外文期刊>Marine biology >Hypoxia-driven selective degradation of cellular proteins in jumbo squids during diel migration to the oxygen minimum zones
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Hypoxia-driven selective degradation of cellular proteins in jumbo squids during diel migration to the oxygen minimum zones

机译：缺氧驱动大乌贼中的细胞蛋白在diel迁移到最低氧区域时的选择性降解

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摘要

The jumbo squid, Dosidicus gigas, is an oceanic top predator in the eastern tropical Pacific that undergoes diel vertical migrations into mesopelagic oxygen minimum zones (OMZs). Besides glycogen breakdown, the pathways of the squid's metabolic (suppression) strategy are poorly understood. Here, juvenile D. gigas were exposed to oxygen levels found in the OMZ off Gulf of California (1 % O_2, 1 kPa at 10 ℃) with the aim to identify, via proteomic tools, eventual anaerobic protein degradation as potential energy source at such depths. Under hypoxia, total protein concentration decreased nonsignificantly from 79.2 ± 12.4 mg g~(-1) wet mass to 74.7 ± 11.7 mg g~(-1) wet mass (p ＞ 0.05). Yet, there was a significant decrease in heat-shock protein (Hsp) 90 and α-actinin contents (p ＜ 0.05). The lower a-actinin concentration at late hypoxia was probably related to decreased protection of the Hsp90 chaperon machinery resulting in increased ubiqui-tination (p ＜ 0.05) and subsequent degradation. Thus, the present findings indicate that D. gigas might degrade, at least under progressing hypoxia, specific mantle proteins anaerobically to increase/maintain anaerobic ATP production and extend hypoxia exposure time. Moreover, the ubiquitin-proteasome system seems to play an important role in hypoxia tolerance, but further investigations are necessary to discover its full potential and pathways.
机译：巨型鱿鱼Dosidicus gigas是东部热带太平洋中的一种海洋顶级捕食者，其diel垂直迁移到中弹性氧气最低限度区域（OMZs）。除了糖原分解外，鱿鱼的代谢（抑制）策略的途径还知之甚少。在这里，少年D. gigas暴露于加利福尼亚湾外的OMZ中发现的氧气水平（1％O_2，在10℃下为1 kPa），目的是通过蛋白质组学工具确定最终的厌氧蛋白质降解是这种蛋白质的潜在能源深度。在缺氧条件下，总蛋白浓度从79.2±12.4 mg g〜（-1）湿重降低到74.7±11.7 mg g〜（-1）湿重无显着降低（p＞ 0.05）。然而，热休克蛋白（Hsp）90和α-肌动蛋白含量显着降低（p ＜0.05）。晚期缺氧时α-肌动蛋白浓度降低可能与对Hsp90分子伴侣机器的保护作用降低有关，导致泛素化增加（p ＜0.05）并随后降解。因此，目前的发现表明，至少在进行中的缺氧条件下，巨球藻可能降解特定的地幔蛋白，以增加/维持厌氧ATP的产生并延长缺氧时间。此外，泛素-蛋白酶体系统似乎在耐缺氧性中起着重要作用，但是需要进一步的研究以发现其全部潜能和途径。

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    《Marine biology》 |2014年第3期|575-584|共10页
  • 作者

    Katja Truebenbach; Goncalo da Costa; Cristina Ribeiro-Silva; Raquel Mesquita Ribeiro; Carlos Cordeiro; Rui Rosa;

  • 作者单位

    Laboratorio Maritimo da Guia, Centre of Oceanography, University of Science in Lisbon, Av. Nossa Senhora do Cabo 939, 2750-374 Cascais, Portugal;

    Department of Chemistry and Biochemistry, University of Science in Lisbon, Campo Grande, 1749-016 Lisbon, Portugal;

    Department of Chemistry and Biochemistry, University of Science in Lisbon, Campo Grande, 1749-016 Lisbon, Portugal;

    Department of Chemistry and Biochemistry, University of Science in Lisbon, Campo Grande, 1749-016 Lisbon, Portugal;

    Department of Chemistry and Biochemistry, University of Science in Lisbon, Campo Grande, 1749-016 Lisbon, Portugal;

    Laboratorio Maritimo da Guia, Centre of Oceanography, University of Science in Lisbon, Av. Nossa Senhora do Cabo 939, 2750-374 Cascais, Portugal;

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