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首页> 外文期刊>Marine biology >A molecular method for the identification of resting eggs of acartiid copepods in the Thau lagoon, France
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A molecular method for the identification of resting eggs of acartiid copepods in the Thau lagoon, France

机译：分子方法鉴定法国塔乌泻湖中的co虫足卵

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摘要

Acartia and Paracartia species, often known to co-occur, can exhibit complex life cycles, including the production of resting eggs. Studying and understanding their population dynamics is hindered by the inability to identify eggs and early developmental stages using morphological techniques. We have developed a simple molecular technique to distinguish between the three species of the Acartiidae family {Acartia clausi, A. discaudata and Paracartia grant) that co-occur in the Thau lagoon (43°25'N; 03°40'E) in southern France. Direct amplification of a partial region of the mitochondrial cytochrome oxidase I gene by polymerase chain reaction and subsequent restriction fragment length polymorphism results in a unique restriction profile for each species. The technique is capable of determining the identity of individual eggs, including resting eggs retrieved from sediment samples, illustrating its application in facilitating population dynamic studies of this ubiquitous and important member of the zooplankton community.
机译：通常已知同时存在的螨和拟寄生虫物种可能表现出复杂的生命周期，包括静息卵的产生。由于无法使用形态学技术识别卵子和早期发育阶段，因此无法研究和了解它们的种群动态。我们已经开发出一种简单的分子技术来区分在塔乌泻湖（43°25'N； 03°40'E）中共存的三种car螨科（Acartia clausi，A。discaudata和Paracartia grant）法国南部。通过聚合酶链反应和随后的限制性片段长度多态性直接扩增线粒体细胞色素氧化酶I基因的部分区域，导致每个物种的独特限制性图谱。该技术能够确定单个卵的身份，包括从沉积物样本中回收的静息卵，从而说明了其在促进浮游动物群落这一无处不在的重要成员的种群动态研究中的应用。

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  • 来源
    《Marine biology》 |2013年第3期|737-742|共6页
  • 作者

    P. K. Lindeque; S. Boyer; D. Bonnet;

  • 作者单位

    Plymouth Marine Laboratory, Prospect Place, West Hoe, Plymouth PL1 3DH, UK;

    Laboratoire EcoSym (Ecologie des Systemes Marins Cotiers), UMR5119, Universite Montpellier 2, CC093, Place Eugene Bataillon, 34095 Montpellier, Cedex 05, France;

    Laboratoire EcoSym (Ecologie des Systemes Marins Cotiers), UMR5119, Universite Montpellier 2, CC093, Place Eugene Bataillon, 34095 Montpellier, Cedex 05, France;

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