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The use of polymerase chain reaction for the identification of sciaenid eggs

机译：聚合酶链反应用于鉴定骨质疏松症卵

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摘要

Traditionally, sciaenid eggs have been identified based on morphological characteristics such as size, number of oil globules and/or pigmentation patterns. Identification of sciaenid eggs by these procedures is time consuming and often inaccurate due to considerable egg size overlap among species. The utilization of molecular techniques for the identification of economically important species has become a fundamental component in ecological studies involving fish eggs and larvae. This study reports the development of a series of both multiplex and individual polymerase chain reactions to identify the eggs of 11 sciaenid species commonly found in the Gulf of Mexico and estuaries near Port Aransas and Corpus Christi, TX, USA. Following method development, the discriminatory power of the assay was first determined with samples from adult fish collected from Aransas and Corpus Christi Bays, Galveston Bay and the lower Laguna Madre in northern Mexico. Most (97%) of these fishes were correctly identified to the level of species. To demonstrate the applicability of the assay, wild fish eggs were collected and analyzed from the Aransas Pass tidal inlet from September through December 2005. During this period, the eggs of four targetrnspecies were positively identified which was in keeping with current knowledge regarding the spawning areas and seasons of these sciaenids based on the presence of mature females, eggs and/or larvae. Future use of this method can provide valuable information to better discriminate spawning sites and seasons of these species.
机译：传统上，已经基于形态特征，例如大小，油球的数量和/或色素沉着模式来鉴定坐骨神经节卵。通过这些方法鉴定坐骨神经痛的鸡蛋是费时的，并且由于物种之间较大的卵大小重叠而常常不准确。利用分子技术鉴定经济上重要的物种已成为涉及鱼卵和幼虫的生态研究的基本组成部分。这项研究报告了一系列多重和单个聚合酶链反应的开发，以鉴定常见于墨西哥湾和美国德克萨斯州阿兰萨斯港和科珀斯克里斯蒂附近的河口的11种神经鞘类物种的卵。随着方法的发展，首先从成年鱼的样品中确定了测定的区分力，这些成鱼是从墨西哥北部的阿兰萨斯和科珀斯克里斯蒂湾，加尔维斯顿湾和下拉古纳马德雷收集的。这些鱼类中的大多数（97％）均已根据物种水平正确鉴定。为了证明该方法的适用性，从2005年9月至2005年12月从Aransas Pass潮汐进口收集并分析了野生鱼卵。在此期间，积极鉴定了四个目标物种的卵，这与对产卵区域的最新了解保持一致以及根据成熟雌性，卵和/或幼虫的存在而出现的这些坐骨神经痛的季节。将来使用此方法可以提供有价值的信息，以更好地区分这些物种的产卵地点和季节。

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  • 来源
    《Marine biology》 |2010年第8期|P.1889-1895|共7页
  • 作者

    Lucia B. Carreon-Martinez; Scott A. Holt; B. Scott Nunez; Cynthia K. Faulk; G. Joan Holt;

  • 作者单位

    Great Lakes Institute for Environmental Research, University of Windsor, Windsor, ON, Canada;

    rnThe University of Texas at Austin Marine Science Institute, Port Aransas, TX, USA;

    rnLee College, Baytown, TX, USA;

    rnThe University of Texas at Austin Marine Science Institute, Port Aransas, TX, USA;

    rnThe University of Texas at Austin Marine Science Institute, Port Aransas, TX, USA;

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