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首页> 外文期刊>Marine biology >Evaluation of the 18S rRNA clone library approach to study the diversity of the macroeukaryotic leaf-epiphytic community of the seagrass Posidonia oceanica (L.) Delile
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Evaluation of the 18S rRNA clone library approach to study the diversity of the macroeukaryotic leaf-epiphytic community of the seagrass Posidonia oceanica (L.) Delile

机译：评价18S rRNA克隆文库方法以研究海草波塞冬草（L.）Delile的大真核叶表生群落的多样性

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The sequence comparisons among genes codifying for the RNA component of the small ribosomal sub-unit (16S rRNA or 18S rRNA) in cellular organisms have been largely used to reconstruct their phylogenies, and hence the identification of taxa by means of a molecular approach. Furthermore, the direct DNA isolation from environmental samples and the PCR amplification of the pool of rRNA genes with the subsequent cloning and sequencing have opened the door to the description of naturally occurring microbial communities independently from any culturing technique or morphological identification. These studies have unveiled an enormous hidden diversity in a wide variety of microbial communities. Our main objective was to evaluate the usefulness of the 18S rRNA gene clone libraries to describe the structure of the macroeukaryotic leaf-epiphytic assemblage of the seagrass Posidonia oceanica, and monitor the changes occurring in different stages of its seasonal succession (winter, spring and summer). To that end, we compared the results of these libraries with those provided by classical microscopy techniques. Among both approaches, the screening of clone libraries rendered the highest number of distinct unitsrnnamed operational phylogenetic units. However, diversity estimates provided by both methods were comparable and rendered the highest Shannon Diversity Index (H') at the end of the succession. The major discrepancies were on the different occurrence of some groups. For example, macroal-gae were the most frequent epiphytes counted by microscopy, whereas metazoa (specially, bryozoa) dominated the clone libraries. Altogether the results indicate that clone libraries constitute an excellent complementary approach to classical microscopy methods. To the best of our knowledge, this is the first attempt to describe a marine macroeukaryotic community using a molecular approach such as the analysis of 18S rRNA gene clone libraries.
机译：在细胞生物中编码小核糖体亚基（16S rRNA或18S rRNA）的RNA成分的基因之间的序列比较已被广泛用于重建其系统发育，并因此通过分子方法鉴定分类群。此外，从环境样品中直接分离DNA以及rRNA基因库的PCR扩增以及随后的克隆和测序为独立于任何培养技术或形态学鉴定自然存在的微生物群落开辟了大门。这些研究揭示了广泛的微生物群落中巨大的隐藏多样性。我们的主要目的是评估18S rRNA基因克隆文库用于描述海草Posidonia oceanica大型真核叶表生组合的结构的有效性，并监测其季节演替的不同阶段（冬季，春季和夏季）的变化。 ）。为此，我们将这些文库的结果与经典显微镜技术提供的结果进行了比较。在这两种方法中，克隆文库的筛选提供了最多数量的被称为操作系统发生单位的不同单位。然而，两种方法提供的多样性估计值是可比的，并且在演替结束时呈现最高的香农多样性指数（H'）。主要差异在于某些群体的发生率不同。例如，宏观显微镜是显微镜下计数最频繁的附生植物，而后生动物（特别是苔藓动物）主导了克隆文库。总之，结果表明克隆文库构成了经典显微镜方法的极佳补充方法。据我们所知，这是首次尝试使用分子方法（例如18S rRNA基因克隆文库分析）描述海洋大型真核生物群落。

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  • 来源
    《Marine biology》 |2009年第9期|1963-1976|共14页
  • 作者

    F. J. Medina-Pons; J. Terrados; A. Lopez-Lopez; P. Yarza; R. Rossello-Mora;

  • 作者单位

    Marine Macrophyte Ecology Group, Ecology and Marine Resources Department, IMEDEA (CSIC-UIB), Miquel Marques 21, 07190 Esporles, Majorca, Spain;

    Marine Macrophyte Ecology Group, Ecology and Marine Resources Department, IMEDEA (CSIC-UIB), Miquel Marques 21, 07190 Esporles, Majorca, Spain;

    Marine Microbiology Group, Ecology and Marine Resources Department, IMEDEA (CSIC-UIB), Miquel Marques 21, 07190 Esporles, Majorca, Spain;

    Marine Microbiology Group, Ecology and Marine Resources Department, IMEDEA (CSIC-UIB), Miquel Marques 21, 07190 Esporles, Majorca, Spain;

    Marine Microbiology Group, Ecology and Marine Resources Department, IMEDEA (CSIC-UIB), Miquel Marques 21, 07190 Esporles, Majorca, Spain;

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