...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Marine biology >Cloning and expression of a tauropine dehydrogenase from the marine sponge Suberites domuncula
【24h】

Cloning and expression of a tauropine dehydrogenase from the marine sponge Suberites domuncula

机译：海洋海绵扁桃体牛磺平脱氢酶的克隆与表达

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The cDNA sequence coding for tauropine dehydrogenase (TaDH) [belonging to the family of opine dehydrogenases] has been determined. Using the demo-sponge Suberites domuncula, we describe for the first time the tauropine dehydrogenase gene (of length 2,992 kb) from a eukaryote, consisting of two introns flanked by three exons. Moreover, two allelic variants have been identified, which are present in the different specimens either in a homozygotic or in a heterozygotic way; the data suggest an intermediary type of heritance. Phylogenetic analyses indicate that S. domuncula TaDH is only distantly related to the opine dehydrogenases from marine invertebrates; rather it comprises high sequence similarity to bacterial ornithine cyclodeaminases (OCD). In addition, expression studies revealed that the steady-state level of TaDH dropped drastically in animals, which had been exposed to elevated aeration. Antibodies raised against the recombinant sponge TaDH were used to demonstrate that S. domuncula expresses high levels of this enzyme in almost all cells. If tissue samples were kept under additional aeration no immuno-signals could be identified. A strong accumulation of the enzyme was seen around the bacteria, existing in bacteriocytes, indicating that under aerobic conditions the bacteria might produce taurine. These data suggest involvement of the sponge TaDH in the final step of the glycolytic pathway, more specifically, in regeneration of NAD(+) under anaerobic conditions. Furthermore, potential mutual influences between bacteria and sponge are discussed, claiming a horizontal gene transfer of the gene from a bacterium to the sponge.
机译：已经确定了编码牛磺碱脱氢酶（TaDH）[属于阿片脱氢酶家族]的cDNA序列。我们使用演示海绵Suberites domuncula，我们第一次描述了真核生物的牛磺平脱氢酶基因（长度为2,992 kb），该基因由两个内含子和三个外显子组成。此外，已经鉴定出两个等位基因变体，它们以纯合或杂合的方式存在于不同的标本中。数据表明是遗传的中间类型。系统发育分析表明，木乃伊TaDH仅与来自海洋无脊椎动物的阿片脱氢酶有很远的联系。相反，它与细菌鸟氨酸环脱氨酶（OCD）具有高度的序列相似性。另外，表达研究表明，动物的TaDH稳态水平急剧下降，而动物暴露于高水平的曝气中。使用针对重组海绵TaDH的抗体来证明domuncula domuncula在几乎所有细胞中均表达高水平的这种酶。如果将组织样品放在另外的通风条件下，则无法识别免疫信号。细菌周围存在大量细菌，存在于细菌细胞中，表明在有氧条件下细菌可能产生牛磺酸。这些数据表明海绵TaDH参与糖酵解途径的最后一步，更具体地说，是在厌氧条件下再生NAD（+）。此外，还讨论了细菌和海绵之间的潜在相互影响，声称该基因从细菌到海绵的水平基因转移。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号