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首页> 外文期刊>Marine biology >Reply to the comment of Fritsch and Karampelas on 'Determination of canthaxanthin in the red coral {Corallium rubrum) from Marseille by HPLC combined with UV and MS detection' by Cvejic et al. (2007) Mar Biol 152:855-862
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Reply to the comment of Fritsch and Karampelas on 'Determination of canthaxanthin in the red coral {Corallium rubrum) from Marseille by HPLC combined with UV and MS detection' by Cvejic et al. (2007) Mar Biol 152:855-862

机译：回答弗里奇和卡兰佩拉斯关于Cvejic等人“通过HPLC结合紫外和质谱检测法测定马赛红珊瑚中的角黄素”的评论。 （2007）Mar Biol 152：855-862

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We have read with interest the comments of Fritsch and Karampelas on our paper entitled "Determination of canthaxanthin in the red coral (Corallium rubrum) from Marseille by HPLC combined with UV and MS detection". The authors show yet unpublished results that they have obtained by Raman spectroscopy on a non-demineralized skeleton of Corallium rubrum. They conclude that "a mixture of 'unsubstituted polyenes' is the most probable cause for the colour of C. rubrum from Marseille or elsewhere". In our paper we used a totally different approach, since we extracted the pigments present within the mineral fraction by decalcifying the axial skeleton and spicules instead of studying the crude skeleton by Raman spectroscopy. We then performed chemical analyses of organic molecules extracted by HPLC combined with UV and MS detection, which is an established reference technique commonly used for the analysis of carotenoids in marine organisms.
机译：我们感兴趣地阅读了Fritsch和Karampelas在题为“通过HPLC与UV和MS检测相结合测定马赛红珊瑚（红珊瑚）中的角黄素中的评论”的评论。作者显示了尚未发表的结果，这些结果是他们通过拉曼光谱法在红-的非去矿化骨架上获得的。他们得出结论：“'未取代的多烯'的混合物是来自马赛或其他地方的红毛衣藻颜色的最可能原因”。在我们的论文中，我们使用了一种完全不同的方法，因为我们通过脱钙轴向骨架和针状物来提取矿物级分中存在的色素，而不是通过拉曼光谱研究粗骨架。然后，我们对通过HPLC提取的有机分子进行了化学分析，并结合了UV和MS检测，这是一种公认​​的参考技术，通常用于分析海洋生物中的类胡萝卜素。

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