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Temporal analysis of population genetic composition in the overexploited Japanese eel Anguilla japonica

机译:过度开发的日本鳗鳗的种群遗传组成的时间分析

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The Japanese eel has dramatically declined in Asia since the 1970s. Over the past two decades, glass eel productivity in Taiwan has decreased, but is highly variable among each year catch, though the cause for this is unknown. The impact of both population decline and instability on genetic diversity, however, is unknown. In this study, we investigated changes in allele frequencies of Japanese eel recruitment events over the past 20 years using six polymorphic microsatellite DNA loci. Specimens of glass eels were collected yearly from a single location in northern Taiwan from 1986 to 2007. Overall genetic differentiation among all samples was very low but significant (F_(ST) = 0.002, P - 0.002), and only 2 out of 120 pairwise tests were significant. The relationship between genetic and temporal distance showed a slight but insignificant correlation (R~2 = 0.03, P = 0.0504). There were no overall significant differences in allelic richness (P = 0.35) or genetic heterozygosity (P = 0.73) among annual recruitment events. No apparent loss of genetic diversity and occurrence of a genetic bottleneck for eel populations were observed. Estimates of the effective population size (N_e) generally exceeded 500, although confidence intervals were very wide. While El Nino /Southern Oscillation (ENSO) events had little impact on genetic diversity, they may account for the annual fluctuation in glass eel catch. These results indicate long-term stability of genetic diversity in the Japanese eel with little evidence for sweepstakes recruitment.
机译:自1970年代以来,日本鳗鱼在亚洲急剧下降。在过去的二十年中,台湾的玻璃鳗产量下降了,但是每年的产量变化很大,尽管其原因尚不清楚。然而,人口下降和不稳定对遗传多样性的影响尚不清楚。在这项研究中,我们使用六个多态微卫星DNA基因座,调查了过去20年日本鳗鱼募集事件的等位基因频率的变化。从1986年到2007年,每年从台湾北部的一个地点收集玻璃鳗标本。所有样品的总体遗传分化非常低,但很显着(F_(ST)= 0.002,P-0.002),在120个配对中只有2个测试很重要。遗传距离与时间距离之间的关系显示出轻微但无关紧要的关系(R〜2 = 0.03,P = 0.0504)。在年度招募事件之间,等位基因丰富度(P = 0.35)或遗传杂合度(P = 0.73)没有总体上的显着差异。没有观察到鳗鱼种群遗传多样性的明显丧失和遗传瓶颈的发生。有效人口规模(N_e)的估计值通常超过500,尽管置信区间非常宽。虽然厄尔尼诺/南方涛动(ENSO)事件对遗传多样性影响很小,但它们可能解释了玻璃鳗捕捞量的年度波动。这些结果表明日本鳗鱼遗传多样性具有长期稳定性,几乎没有招募抽奖的证据。

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