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首页> 外文期刊>Marine biology >Levels of intra-host and temporal sequence variation in a large CO1 sub-units from Anisakis simplex sensu stricto (Rudolphi 1809) (Nematoda: Anisakisdae): implications for fisheries management
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Levels of intra-host and temporal sequence variation in a large CO1 sub-units from Anisakis simplex sensu stricto (Rudolphi 1809) (Nematoda: Anisakisdae): implications for fisheries management

机译：Anisakis simplex sensu stricto（Rudolphi 1809）（Nematoda：Anisakisdae）的一个大型CO1亚基中寄主内和时间序列变异的水平：对渔业管理的影响

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This paper is the first to address the suitability and potential of the cytochrome oxidase-1 (CO1) region of the parasitic marine nematode Anisakis simplex sensu stricto as a genetic marker. A. simplex s.s. is an ubiquitous parasite of many marine organisms and has been used as a 'biological tag' for population studies of pelagic fish stocks. The CO1 marker informs not only about nematode population structure but also about its hosts. The large CO1 sub-unit (～800 bp) was analysed from third stage larvae of A. simplex s.s. from Atlantic herring, Clupea harengus L. caught off the north-west coast of Scotland. In total 161 A. simplex s.s. CO1 sequences were analysed from 37 herring that represented three spawning periods over 2 years. Overall very high haplotype and low nucleotide diversities were observed (h = 0.997 and π = 0.008, respectively). These results are in keeping with studies investigating parasitic nematodes of ungulates and are symptomatic of the high rate of substitutions accumulated by mtDNA and effective dispersal strategies of the parasite. The Tamura-Nei I + Γ (Γ = 1.2243) model of nucleotide substitution best suited the present data which were dominated by a high thymine bias and associated transitions. Large within population differences were highlighted by hier-archal AMOVAs with little variation related to spawning events or years which may indicate localised temporal stability. Temporal homogeneity in the CO1 gene coupled with the ubiquitous and widespread nature of the parasite indicates both the potential and limitations for its incorporation in stock-separation studies of its hosts.
机译：这篇论文是第一个研究寄生性海洋线虫Anisakis simplex sensu stricto作为遗传标记的细胞色素氧化酶1（CO1）区的适用性和潜力的论文。 A.单纯形是许多海洋生物无处不在的寄生虫，已被用作远洋鱼类种群种群研究的“生物标签”。 CO1标记不仅告知线虫种群结构，还告知其宿主。从单纯根霉的第三阶段幼虫中分析了大的CO1亚基（〜800 bp）。来自大西洋鲱鱼的Clupea harengus L.在苏格兰西北海岸附近捕捞。总共161 A. simplex s.s.从代表2个年中的三个产卵期的37个鲱鱼中分析了CO1序列。总体上观察到非常高的单倍型和低核苷酸多样性（分别为h = 0.997和π= 0.008）。这些结果与研究有蹄类动物的寄生线虫的研究相符，并且是mtDNA积累的高替代率和有效的寄生虫扩散策略的征兆。核苷酸取代的Tamura-Nei I +Γ（Γ= 1.2243）模型最适合当前数据，该模型主要由高胸腺嘧啶偏差和相关的跃迁控制。层级AMOVA突出显示了较大的群体差异，与产卵事件或年份相关的变化很小，这可能表明局部时间稳定。 CO1基因的时间同质性与寄生虫的普遍性和普遍性相结合，表明将其纳入宿主宿主分离研究中的潜力和局限性。

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