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首页> 外文期刊>Marine biology >Effects of salinity on the clam Chamelea gallina. Part I: alterations in immune responses
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Effects of salinity on the clam Chamelea gallina. Part I: alterations in immune responses

机译：盐度对蛤ChaChamelea gallina的影响。第一部分：免疫反应的改变

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In the present study, the effects of differing salinities on some important functional responses of haemocytes from the clam, Chamelea gallina, were investigated. The animals were kept for 7 days at 28‰ (hyposalinity), 34‰ (control) and 40‰ salinity (hypersalinity), and total haemocyte count (THC), haemocyte volume, phagocytosis, lysozyme-like activity (in both haemocyte lysate and cell-free haemol-ymph) were measured. The survival-in-air test was also performed. Clams kept at 28‰ showed significantly increased THC with respect to animals kept at 34 and 40‰. The analysis of haemocyte size frequency distribution highlighted that in clams kept at 28‰ the haemocyte fraction of about 5 urn in diameter and 50-100 femtolitre in volume increased markedly. Conversely, in animals kept at 40‰ an increase was observed in the haemocyte fraction having about 8-10 urn diameter and 400-500 femtolitre volume. Higher phagocytic activity was recorded in haemocytes from control clams, with respect to that of clams kept at 28 and 34‰. Lysozyme-like activity in haemocyte lysate was shown to increase significantly in animals kept at 28‰ with respect to that of clams kept at 40‰, whereas enzyme activity in cell-free haemolymph from clams kept at 34‰ was significantly higher with respect to that of clams maintained at 40‰. A relationship between phagocytosis and lysozyme secretion is suggested. The resistance to air exposure of clams kept at 28 and 40‰ was shown to decrease significantly; LT_(50) values fell from 7 days in clams kept at 34‰ to 4 and 5 days in those kept at 28 and 40‰, respectively. Results demonstrated that salinity values far from 34‰ affects the functional responses of haemocytes and reduce the resistance of clams to exposure to air.
机译：在本研究中，研究了不同盐度对蛤Cha Chamelea gallina血细胞某些重要功能反应的影响。将动物分别保持在28‰（低盐度），34‰（对照）和40‰盐度（高盐度）下7天，总血细胞计数（THC），血细胞体积，吞噬作用，溶菌酶样活性（在血细胞裂解液和测量无细胞血红素-淋巴）。还进行了空中生存测试。保持在28‰的蛤showed相对于保持在34和40‰的动物，THC显着增加。对血红细胞大小频率分布的分析表明，在蛤维持在28‰时，血红细胞部分的直径大约为5 um，体积为50-100毫微升。相反，在保持在40‰的动物中，观察到直径约8至10 n，体积为400至500毫托的血细胞比例增加。对照蛤的血细胞中吞噬活性更高，相对于保持在28和34‰的蛤。与保持在40‰的蛤相比，在保持28‰的动物中，血细胞裂解液中的溶菌酶样活性显着增加，而保持在34‰的蛤的无细胞血淋巴中的酶活性明显高于保持在40‰的蛤。蛤维持在40‰。吞噬作用与溶菌酶分泌之间存在联系。蛤类的暴露于空气的抵抗力保持在28和40‰，显示出显着降低。 LT_（50）值从保持在34‰的蛤的7天下降到保持在28‰和40‰的蛤的4天和5天。结果表明，盐度值远非34‰会影响血细胞的功能反应，并降低蛤to对空气的抵抗力。

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