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Isolation and characterization of two actins of the Pacific white shrimp, Litopenaeus vannamei

机译：太平洋白虾南美白对虾的两种肌动蛋白的分离和鉴定

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Two actin genes named actinT1 and actinT2 were isolated and sequenced from the Pacific white shrimp, Litopenaeus vannamei, by screening from a shrimp eyestalk cDNA library. L. vannamei actinT1 cDNA has a 1,128-bp open reading frame encoding for 376 amino acids while L. vannamei actinT1 cDNA has a 1,131-bp open reading frame coding for 377 amino acids. Alignment of the actinT1 and actinT2 cDNA sequences showed that these two actin genes share a sequence identity of 86% at amino acid residues. When compared with actins of several other invertebrate and vertebrate species, the nucleotide sequence of actinT1 is highly homologous (97-100%) with beta-actins, while actinT1 shares 86-95% identity with alpha-actins on the nucleotide level. Phylogenetic analysis and BLAST searches indicated that the ActinT1 protein is identical to crustacean beta-actins, while the ActinT2 protein is highly homologous to crustacean alpha-actins. Constitutive expression of the actinT1 and actinT2 genes were detected by RT-PCR in all adult shrimp organs, including brain, eye-stalk, gill, heart, hemolymph, hepatopancreas, muscle, swimming legs, and stomach, as well as in the shrimp zygote, nauplius, and mysis life stages. These data will facilitate attempts to clone and identify more shrimp genes and constitutive shrimp promoters.
机译：通过从虾的视线cDNA文库中筛选，从太平洋白对虾凡纳滨对虾中分离并测序了两个名为actinT1和actinT2的肌动蛋白基因，并对其进行了测序。凡纳哥肌动蛋白T1 cDNA具有1,128-bp的开放阅读框，编码376个氨基酸，而凡纳哥乳酸肌动蛋白T1 cDNA具有1,131-bp的开放阅读框，编码377个氨基酸。肌动蛋白T1和肌动蛋白T2 cDNA序列的比对表明，这两个肌动蛋白基因在氨基酸残基上具有86％的序列同一性。与其他几种无脊椎动物和脊椎动物的肌动蛋白相比，肌动蛋白T1的核苷酸序列与β-肌动蛋白高度同源（97-100％），而肌动蛋白T1在核苷酸水平上与α-肌动蛋白具有86-95％的同一性。系统发育分析和BLAST搜索表明，ActinT1蛋白与甲壳类β-肌动蛋白相同，而ActinT2蛋白与甲壳类α-肌动蛋白高度同源。通过RT-PCR在所有成年虾器官中检测到actinT1和actinT2基因的组成型表达，包括大脑，眼柄，腮，心脏，血淋巴，肝胰腺，肌肉，游泳的腿和胃，以及虾合子，无节幼体和mysis生命阶段。这些数据将有助于尝试克隆和鉴定更多虾基因和组成型虾启动子。

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