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首页> 外文期刊>Marine biology >Assessment of estrogenic chemicals using an estrogen receptor α (ERα)- and ERβ-mediated reporter gene assay in fish
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Assessment of estrogenic chemicals using an estrogen receptor α (ERα)- and ERβ-mediated reporter gene assay in fish

机译：用雌激素受体α（ERα）和ERβ介导的报告基因对鱼类进行雌激素化学物质评估

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摘要

In vitro reporter gene assays using vertebrate cell lines or yeast have been used for assessment of the estrogenic chemicals. However, estrogen receptor α (ERα )- and ERβ-mediated reporter gene system in fish has yet to be developed. In the present study, we developed an ERα - and ERβ-mediated reporter gene assay in fish and estimated estrogenic activities of 17β-estradiol (E2), p-nonylphenol (NP), bisphenol A (BPA), p,p′-DDE, and genistein (Gen) using the in vitro reporter assay. The activity was intensely induced by transfection with either ERα or ERβ expression plasmid under E2 or Gen administration, whereas it was significantly induced by transfection with ERα expression plasmid, but not with ERβ expression plasmid under NP administration. On the other hand, the activity was induced more intensely by transfection with ERα expression plasmid than ERβ expression plasmid under BPA or p,p′-DDE administration. These results indicate that there are obvious differences in the activity through ERα and ERβ among the estrogenic chemicals examined in vitro. Thus, the in vitro reporter assay provides an excellent system for elucidating the action mechanism of estrogenic chemicals in fishes.
机译：使用脊椎动物细胞系或酵母的体外报道基因测定已用于评估雌激素化学物质。然而，鱼类中雌激素受体α（ERα）和ERβ介导的报告基因系统尚未开发。在本研究中，我们开发了鱼类中ERα和ERβ介导的报告基因测定方法，并估算了17β-雌二醇（E2），对壬基酚（NP），双酚A（BPA），p，p'-DDE的雌激素活性，和染料木黄酮（Gen）使用体外报告基因检测。通过在E2或Gen施用ERα或ERβ表达质粒转染可强烈诱导活性，而通过ERα表达质粒转染可显着诱导活性，而在NP施用可不经ERβ表达质粒转染。另一方面，在BPA或p，p′-DDE给药下，通过ERα表达质粒转染比ERβ表达质粒更强烈地诱导活性。这些结果表明，在体外检查的雌激素化合物中，通过ERα和ERβ的活性存在明显差异。因此，体外报告基因测定为阐明鱼类中雌激素化学物质的作用机理提供了一个极好的系统。

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