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首页> 外文期刊>Marine biology >Evidence that the rotifer Brachionus plicatilis is not an osmoconformer
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Evidence that the rotifer Brachionus plicatilis is not an osmoconformer

机译：轮虫Brachionus plicatilis不是渗透剂的证据

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The rotifer Brachionus plicatilis is euryhaline ( growing between 2 and 97 ppt) and has previously been considered an osmoconformer. We suggest that B. plicatilis is an osmoregulator, exhibiting a pattern of Na+/ K+ ATPase activity in response to salinity consistent with that of other osmoregulating euryhaline invertebrates. To examine salinity tolerance, growth rates between 5 and 60 ppt were determined. The activity of Na+/ K+ ATPase was examined, over the same range of salinities, by measuring ATPase activity in rotifer homogenates in the presence and absence of a Na+/ K+ ATPase inhibitor. Maximum specific growth rate (0.95 day(-1)) occurred at 16 ppt, highest mean amictic eggs per female (1.41) occurred at 20 ppt, and both parameters decreased rapidly as salinity increased. Egg development time was constant with salinity at 0.92 days. The activity of Na+/ K+ ATPase per milligram protein increased from 3.9 mu mol h(-1) at 5 ppt to 6.8 mu mol h(-1) at 50 ppt and accounted for 15 and 30% of total ATPase activity, respectively. We suggest that these observations are consistent with increasing stress at high salinities and the occurrence of a hypo-osmoregulatory response. Given the high ATP consumption of Na+/ K+ ATPase at high salinities, it is possible that a proportion of the corresponding decreases in growth rate and egg production are a direct cost of regulation.
机译：轮虫Brachionus plicatilis是euryhaline（生长在2至97 ppt之间），以前被认为是渗透压适形者。我们建议B. plicatilis是一种渗透调节剂，与其他渗透调节性欧莱雅无脊椎动物的盐度一致，表现出Na + / K + ATPase活性模式。为了检查耐盐性，确定了5至60 ppt之间的增长率。通过在存在和不存在Na + / K + ATPase抑制剂的情况下，通过测量轮虫匀浆中的ATPase活性，在相同盐度范围内检查Na + / K + ATPase的活性。最大比生长速率（0.95天（-1））出现在16 ppt处，最高雌性平均虫卵（1.41）出现在20 ppt处，并且两个参数随着盐度的增加而迅速降低。卵的发育时间恒定，盐度为0.92天。每毫克蛋白Na + / K + ATPase的活性从5 ppt的3.9μmol h（-1）增加到50 ppt的6.8μmol h（-1），分别占总ATPase活性的15％和30％。我们建议这些观察结果与高盐度下压力增加以及渗透调节反应减退的发生是一致的。鉴于在高盐度下Na + / K + ATPase的ATP消耗量很高，相应比例的生长速率和产蛋量下降可能是调节的直接成本。

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