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首页> 外文期刊>Marine biology >Development and characterization of complex particles for delivery of amino acids to early marine fish larvae
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Development and characterization of complex particles for delivery of amino acids to early marine fish larvae

机译：复杂的颗粒的开发和表征，用于向早期海鱼幼体输送氨基酸

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Complex particles (CP), zein microbound particles and lipid-spray beads (LSB) were prepared and their performances were compared for delivering glycine and a mixture of free amino acids (FAA; alanine, glycine, leucine, serine and tyrosine) to early fish larvae. Measures of performances of microparticles included inclusion, encapsulation, retention and delivery efficiencies in addition to T50 ( time to 50% retention) values. CP were prepared containing LSB and a defined dietary mixture that were bound together with zein. CP had significantly higher retention and delivery efficiencies for FAA compared to those of zein microbound particles. Free LSB had higher retention efficiencies for particulate glycine compared with CP, possibly due to differences in suspension characteristics. Free LSB clumped and floated when suspended in water, negatively afecting their acceptability by fish larvae; therefore, LSB should be incorporated into CP for more effective delivery of amino acids. There was a significant inverse correlation between retention efficiencies and solubilities of FAA encapsulated in CP. After 1 h of aqueous suspension, highest retention (44%) and delivery efficiencies (20.3 mg tyrosine g(-1) particle) were achieved with tyrosine. T50 values indicated 50% of the initial tyrosine in CP was still available after 36.7 min of suspension in water. Furthermore, CP can deliver FAA to marine fish larvae based on the results of feeding studies in which CP were digested by 3-day-old clown fish larvae; therefore, this particle type could be a valuable tool in studies of larval fish nutrition.
机译：制备了复合颗粒（CP），玉米醇溶蛋白微结合颗粒和脂质喷雾珠（LSB），并比较了它们向早期鱼类输送甘氨酸和游离氨基酸混合物（FAA；丙氨酸，甘氨酸，亮氨酸，丝氨酸和酪氨酸）的性能。幼虫。微粒性能的度量标准包括T50（保留时间为50％的时间）值以外的包容性，包封性，保留率和传递效率。制备包含LSB和与玉米蛋白结合在一起的确定的饮食混合物的CP。与玉米醇溶蛋白微结合颗粒相比，CP对FAA的保留和递送效率明显更高。与CP相比，游离LSB对颗粒状甘氨酸的保留效率更高，这可能是由于悬浮特性的差异所致。游离的LSB悬浮在水中时会结块并漂浮，这会对鱼幼体的接受性产生负面影响；因此，应将LSB掺入CP中以更有效地递送氨基酸。在CP中封装的FAA的保留效率和溶解度之间存在显着的负相关关系。水悬浮液1小时后，酪氨酸可实现最高保留率（44％）和输送效率（20.3 mg酪氨酸g（-1）颗粒）。 T50值表明在水中悬浮36.7分钟后，CP中50％的初始酪氨酸仍然可用。此外，根据饲喂研究的结果，CP被3天龄的小丑鱼幼虫消化，CP可以将FAA输送到海鱼幼虫中。因此，这种颗粒类型可能是研究幼鱼营养的有价值的工具。

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