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首页> 外文期刊>Marine biology >Correlating gene expression with larval competence, and the effect of age and parentage on metamorphosis in the tropical abalone Haliotis asinina
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Correlating gene expression with larval competence, and the effect of age and parentage on metamorphosis in the tropical abalone Haliotis asinina

机译：将基因表达与幼虫能力相关，以及年龄和亲子关系对热带鲍鱼鲍鱼盐沼变态的影响

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摘要

The non-geniculate crustose coralline alga (CCA) Mastophora pacifica can induce the metamorphosis of competent Haliotis asinina (Vetigastropoda) larvae. The ability to respond to this natural cue varies considerably with larval age, with a higher proportion of older larvae (e.g. 90 h) able to metamorphose in response to M. pacifica than younger larvae (e.g. 66 h). Here we document the variation in time to acquisition of competence within a larval age class. For example, after 18 h of exposure to M. pacifica, approximately 15 and 36% of 84 and 90-h-old PL asinina larvae had initiated metamorphosis, respectively. This age-dependent response to M. pacifica is also observed when different aged larvae are exposed to CCA for varying periods. A higher proportion of older larvae require shorter periods of exposure to CCA than younger larvae in order to initiate metamorphosis. In this experiment, as in the previous, a small proportion of young larvae were able to respond to brief periods of CCA exposure, suggesting that they had developed the same state of competency as the majority of their older counterparts. Comparisons of the proportions of larvae undergoing metamorphosis between families reveals that parentage also has a significant (P < 0.05) affect on whether an individual will initiate metamorphosis at a given age. These familial differences are more pronounced when younger, largely pre-competent larvae (i.e. 66 h old) are exposed to M. pacifica, with proportions of larvae undergoing metamorphosis differing by as much as 10 fold between families. As these data suggest that variation in the rate of development of the competent state has a genetic basis, and as a first step towards identifying the molecular basis to this variation, we have identified numerous genes that are differentially expressed later in larval development using a differential display approach. Spatial expression analysis of these genes suggests that they may be directly involved in the acquisition of competence, or may play a functional role in the postlarva following metamorphosis.
机译：非膝状硬壳珊瑚藻（CCA）太平洋扁线菌（Mastophora pacifica）可以诱导感受态拟盐单胞菌（Vetigastropoda）幼虫的变态。响应这种自然提示的能力随幼虫年龄的变化而变化很大，能够响应太平洋支原体变态的较大年龄的幼虫（例如90小时）比年轻的幼虫（例如66小时）更高。在这里，我们记录了幼虫年龄段内获得能力的时间变化。例如，在暴露于太平洋支原体18小时后，分别有84％和90小时的PL asinina幼虫分别约有15％和36％引发了变态。当不同年龄的幼虫在不同时期接触CCA时，也会观察到这种对太平洋分枝杆菌的年龄依赖性反应。为了引发变态，年龄较大的幼虫比年龄较小的幼虫需要更短的接触CCA的时间。与以前一样，在该实验中，一小部分幼虫能够对短暂的CCA暴露做出反应，这表明他们与大多数年长幼虫具有相同的能力水平。比较不同家庭之间发生变态的幼虫的比例，发现父母身份也对个体是否会在给定年龄引发变态产生重大影响（P <0.05）。当年轻的，具有超能力的幼虫（即66小时大）的幼虫暴露于太平洋分枝杆菌时，这些家族差异更加明显，而幼虫经历变态的比例在家庭之间相差多达10倍。这些数据表明，感受态的发育速率的变异具有遗传基础，并且作为鉴定这种变异的分子基础的第一步，我们已经鉴定了许多基因，这些基因在后期的幼虫发育中使用差异显示方式。这些基因的空间表达分析表明，它们可能直接参与能力的获得，或者可能在变态后的幼虫后起功能性作用。

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