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首页> 外文期刊>Marine biology >Pacific harbor seals (Phoca vitulina) and salmon: genetics presents hard numbers for elucidating predator-prey dynamics
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Pacific harbor seals (Phoca vitulina) and salmon: genetics presents hard numbers for elucidating predator-prey dynamics

机译：太平洋海豹（Phoca vitulina）和鲑鱼：遗传学提供了难以解释的捕食者-猎物动态的数字

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摘要

We used a PCR-based species test and micro-satellite genotyping to determine salmonid species composition and quantity of Chinook among Pacific harbor seal (Phoca vitulina) prey. Tests were applied to DNA extracted from all salmonid bones recovered from scat (fecal) samples. Condition of bone samples and quantity of DNA varied substantially. Although DNA extraction and species identification was only successful for just over half of the bone samples, 93% of all scat samples had bones that were identified to species. Most often only a single salmonid prey species was evident within a scat sample (39% exclusively coho and 46% exclusively Chinook) but 13% contained bones from more than one species of salmonid. For scat samples that contained Chinook bones, 68% had skeletal remains from the same fish and 32% had bones from more than one individual Chinook, varying in number from two to four individuals per scat. In one case, bones from one individual Chinook were recovered in three different scat samples.
机译：我们使用了基于PCR的物种测试和微卫星基因分型，以确定太平洋斑海豹（Phoca vitulina）猎物中鲑鱼的奇努克族物种组成和数量。对从粪便（粪便）样品中回收的所有鲑鱼骨提取的DNA进行了测试。骨骼样品的状况和DNA的数量差异很大。尽管仅对一半以上的骨骼样本进行了DNA提取和物种鉴定才成功，但所有粪便样本中的93％都具有对物种进行鉴定的骨骼。大多数情况下，粪便样本中只显示出一个单一的鲑鱼捕食物种（仅39％的美洲狮和46％的奇努克犬），但13％的鲑鱼的骨头来自一种以上的鲑鱼。对于含有奇努克人骨骼的粪便样本，有68％的人从同一条鱼身上留下了骨骼，而有32％的人有不止一只奇努克人的骨骼，每只粪便中有2到4个人不等。在一种情况下，在三个不同的粪便样本中回收了一个个体奇努克人的骨头。

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