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Kinetics of trace metal accumulation and excretion in the polychaete Nereis diversicolor

机译:多斑杂色猪笼草中痕量金属积累和排泄的动力学

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The comparative kinetics of the accumulation of the trace metals copper, zinc and cadmium have been measured in the estuarine burrowing polychaete worm Nereis (Hediste) diversicolor from two sites: (a) a metal-rich site, Restronguet Creek, Cornwall, UK, which hosts a copper- and zinc-tolerant population of worms, and (b) the Blackwater estuary, Essex, UK as a control site. A sediment transfer experiment showed that the Blackwater worms responded to the increased copper bioavail-ability in Restronguet Creek sediment by accumulating significantly increasing copper concentrations over 50 days. The Restronguet Creek worms showed no significant change in copper concentration over 50 days in sediment from either site or in sand. Nevertheless, electron microscopy showed that some Restronguet Creek worms do appear to excrete accumulated copper, probably in association with renewal of the cuticle over a long time scale. The Blackwater worms did not accumulate extra zinc from the zinc-rich Restronguet Creek sediment, in probable reflection of the regulation of body zinc concentration by N. diversicolor. Radiolabelled zinc and cadmium were accumulated from labelled sediment and labelled solution by worms from both sites. The rate of uptake of labelled zinc from sediment was significantly greater in the Restronguet Creek worms, as was the rate of uptake of labelled cadmium from 10 μg l~(-1) dissolved exposure; other rates of uptake did not differ between populations. Mucus, which is secreted by Restronguet Creek worms in response to enhanced copper exposure, adsorbed very small proportions of zinc and cadmium present in solution, indicating that the mucus does not act as an adsorption barrier against excessive metal uptake by these worms.
机译:已在两个地点的河口洞穴多毛蠕虫Nereis(Hediste)杂色虫中测量了痕量金属铜,锌和镉的累积动力学,其中两个地点:(a)富含金属的地点,英国康沃尔郡的Restronguet Creek。拥有铜和锌耐受的蠕虫种群,并且(b)英国埃塞克斯的黑水河口为控制点。沉积物迁移实验表明,黑水蠕虫通过在50天内积累了明显增加的铜浓度,从而对Restronguet Creek沉积物中铜的生物利用度提高做出了响应。 Restronguet Creek蠕虫在过去50天的时间里,无论是地点还是沙地中的沉积物,铜浓度均无明显变化。然而,电子显微镜显示,某些Restronguet Creek蠕虫确实会排泄积聚的铜,这可能与长时间表皮的更新有关。黑水蠕虫未从富锌的Restronguet Creek沉积物中积聚多余的锌,这可能反映了杂色猪笼草对体内锌浓度的调节。放射性标记的锌和镉从蠕虫的两个部位从标记的沉积物和标记的溶液中积累。在Restronguet Creek蠕虫中,从沉积物中摄取标记的锌的比率显着更高,从10μgl〜(-1)的溶解暴露中摄取的标记的镉的比率也明显更高。人群之间的其他摄取率没有差异。粘液是Restronguet Creek蠕虫因铜暴露增加而分泌的粘液,其吸附溶液中存在的锌和镉的比例很小,这表明粘液并不充当阻止这些蠕虫过度吸收金属的吸附屏障。

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