...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Marine biology >Within- and among-leaf variations in photo-physiological functions, gene expression and DNA methylation patterns in the large-sized seagrass Posidonia oceanica
【24h】

Within- and among-leaf variations in photo-physiological functions, gene expression and DNA methylation patterns in the large-sized seagrass Posidonia oceanica

机译：大型海草波塞冬虫的光生理功能，基因表达和DNA甲基化模式的叶内和叶间变化

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The knowledge of how molecular functions vary in relation to developmental and environmental cues within and among seagrass leaves is scarce in comparison with terrestrial angiosperms. This strongly limits the mechanistic understanding of photosynthetic development and light acclimation processes in seagrasses, besides having fundamental methodological implications when small leaf sections are utilized as a proxy for assessing the photosynthetic performance and molecular responses to environmental changes for the whole plant. Here, the expression gradients of genes associated with key plant metabolic processes (i.e. photosynthesis, energy dissipation mechanisms, stress response and programmed cell death) were determined, for the first time, in three segments (i.e. basal, medium and high) along the longitudinal axis of three ranked leaves (i.e. leaf 1, 2 and 3) in the large-sized seagrass Posidonia oceanica. The evaluation of major shifts in gene expression paralleled the analysis of photo-physiological properties and global DNA methylation level of the different leaf sections. Photo-physiological and molecular results converged in suggesting that the within-leaf (vertical) gradient was stronger than the leaf-rank (horizontal) gradient, likely reflecting the sharp irradiance attenuation occurring inside the complex canopy formed by this species. Specific correlations between target gene expression and photo-physiological measurements were found, providing a first description of molecular rearrangements underlying the differential photosynthetic performance and light acclimation capacity of seagrass leaves. DNA methylation varied with tissue age, being higher in the youngest and oldest leaf sections, while decreasing in intermediate tissues. We interpreted such changes as a consequence of the interplay between developmental and light cues.
机译：与陆生被子植物相比，关于海草叶片内部和之中分子功能如何与发育和环境线索有关的知识很少。这严重限制了对海草光合作用发展和光适应过程的机械理解，除了在使用小叶片切片作为评估整个植物的光合作用性能和对环境变化的分子响应时，还具有根本的方法学意义。在这里，首次确定了与植物主要代谢过程（即光合作用，能量耗散机制，胁迫反应和程序性细胞死亡）相关的基因的表达梯度，该基因的表达沿纵向分为三个部分（基础，中和高）大型海草波塞冬大洋洲中三个排列的叶子（即叶子1、2和3）的轴。基因表达的主要变化的评估与不同叶片的光生理特性和总体DNA甲基化水平的分析相平行。光生理学和分子学结果的融合表明，叶内（垂直）梯度比叶秩（水平）梯度强，这可能反映了该物种形成的复杂冠层内部急剧的辐照衰减。发现了靶基因表达与光生理学测量之间的特定相关性，为海草叶的差异光合性能和光适应能力提供了分子重排的第一个描述。 DNA甲基化随组织年龄的变化而变化，在最年轻和最老的叶片中较高，而在中间组织中则较低。我们将这种变化解释为发育和光暗示之间相互作用的结果。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号