...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Marine Biotechnology >Construction and Preliminary Analysis of a Deep-Sea Sediment Metagenomic Fosmid Library from Qiongdongnan Basin, South China Sea
【24h】

Construction and Preliminary Analysis of a Deep-Sea Sediment Metagenomic Fosmid Library from Qiongdongnan Basin, South China Sea

机译：南海琼东南盆地深海沉积物基因组化石文库的构建与初步分析

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Preliminary characterization of the microbial phylogeny and metabolic potential of a deep-sea sediment sample from the Qiongdongnan Basin, South China Sea, was carried out using a metagenomic library approach. An effective and rapid method of DNA isolation, purification, and library construction was used resulting in approximately 200,000 clones with an average insert size of about 36 kb. End sequencing of 600 individual clones from the fosmid library generated 1,051 sequences with an average sequence length of 619 bp. Phylogenetic ascription indicated that this library was dominated by Bacteria, predominately Proteobacteria, though Planctomycetes were also relatively abundant. Sulfate-reducing and anaerobic ammonium-oxidizing bacteria, which play important roles in the cycling of sedimentary nutrients, were abundant in the library. Cluster of orthologous groups category analysis showed that most of the genes contained in the end sequences were related to metabolism, and with cellular processes and signaling. Functional groups assigned by SEED (subsystems-based annotations) highlighted the existence of ‘one-carbon’ metabolism within this community as well as identifying functional genes involved in methanogenesis. Furthermore, diverse genes involved in the biodegradation of xenobiotics were found using Kyoto Encyclopedia of Genes and Genomes metabolic pathway analysis.
机译：使用宏基因组库方法对南海琼东南盆地深海沉积物样品的微生物系统发育和代谢潜力进行了初步表征。使用了一种有效，快速的DNA分离，纯化和文库构建方法，产生了大约200,000个克隆，平均插入片段大小约为36 kb。 fosmid文库中的600个克隆的末端测序产生了1,051个序列，平均序列长度为619 bp。系统发育归因表明该文库主要由细菌，主要是Proteobacteria，尽管Planctomycetes也相对丰富。减少硫酸盐和厌氧铵氧化细菌在沉积养分循环中起重要作用，在图书馆中非常丰富。直系同源群分类分析的簇显示，末端序列中包含的大多数基因与代谢，细胞过程和信号传导有关。 SEED（基于子系统的注释）分配的功能组强调了该社区内“一碳”代谢的存在，并确定了参与甲烷生成的功能基因。此外，使用《京都基因百科全书》和基因组代谢途径分析发现了与异种生物降解有关的多种基因。

著录项

  • 来源
    《Marine Biotechnology》 |2010年第6期|p.719-727|共9页
  • 作者

    Yongfei Hu; Chengzhang Fu; Yeshi Yin; Gong Cheng; Fang Lei; Xi Yang; Jing Li; Elizabeth Jane Ashforth; Lixin Zhang; Baoli Zhu;

  • 作者单位

    Institute of Microbiology, Chinese Academy of Sciences, No.1 West Beichen Road, Chaoyang District, Beijing, 100101, People’s Republic of China;

    Institute of Microbiology, Chinese Academy of Sciences, No.1 West Beichen Road, Chaoyang District, Beijing, 100101, People’s Republic of China;

    Institute of Microbiology, Chinese Academy of Sciences, No.1 West Beichen Road, Chaoyang District, Beijing, 100101, People’s Republic of China;

    Institute of Microbiology, Chinese Academy of Sciences, No.1 West Beichen Road, Chaoyang District, Beijing, 100101, People’s Republic of China;

    Institute of Microbiology, Chinese Academy of Sciences, No.1 West Beichen Road, Chaoyang District, Beijing, 100101, People’s Republic of China;

    Institute of Microbiology, Chinese Academy of Sciences, No.1 West Beichen Road, Chaoyang District, Beijing, 100101, People’s Republic of China;

    Institute of Microbiology, Chinese Aca;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Deep-sea sediment; Metagenomics; Fosmid library; End sequences; Methane hydrate;

    机译：深海沉积物;宏组学;粘粒文库;末端序列;甲烷水合物;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号