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首页> 外文期刊>Marine Chemistry >The influence of arsenate and phosphate exposure on arsenic uptake, metabolism and species formation in the marine phytoplankton Dunaliella tertiolecta
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The influence of arsenate and phosphate exposure on arsenic uptake, metabolism and species formation in the marine phytoplankton Dunaliella tertiolecta

机译：砷和磷酸盐的暴露对海洋浮游植物杜氏盐藻中砷吸收，代谢和物种形成的影响

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摘要

The influence of As(Ⅴ) (50 μg L~~(-1)&2)μgL~(-1))andPO_4~(3-) (3 mgL~(-1)&0.12 mg L~(-1)) exposures on arsenic cycling by the marine phytoplankton Dunaliella tertiolecta was investigated both separately and in combination. Total arsenic concentrations in D. tertiolecta tissue ranged from 8 to 10 μg g~(-1) when exposed to 50 μg L~(-1) As( V), and 2-4 μg g~(-1) when exposed to 2 μg L~(-1) As(Ⅴ), with P0_4~(3-) exposure having little to no influence on total arsenic concentrations. Arsenic was evenly distributed (≈33%) between the three major biochemical cell fractions (lipid, water, residue) in cultures exposed to high As(Ⅴ):high PO_4~(3-) concentrations. Under low As(Ⅴ):high PO_4~(3-) exposures arsenic was predominantly lipid-soluble (40-55%) and residue bound (30-40%). When exposed to high As(Ⅴ):low PO_4~(3-) concentrations arsenic was predominantly residue bound (50-70%) and lipid-soluble (20-40%), whilst under low As(Ⅴ):low PO_4~(3-) exposures arsenic was predominantly residue bound (75-80%). As(Ⅴ) was the dominant arsenic species present in the water-soluble cell fractions of all D. tertiolecta cultures. Under high PO_4~(3-) concentrations As(Ⅴ) accounted for greater than 93% of the extractable water-soluble arsenic with As(Ⅲ) the only other species detected. Conversely, under low PO_4~(3-) concentrations As(Ⅴ) accounted for between 66 and 71% of the extractable arsenic with As(Ⅲ) (16-26%) and PO_4-riboside (8-13%) present. Under high As(Ⅴ):high PO_4~(3-) concentrations As(Ⅴ) was the major arsenic species in hydrolysed lipid extracts accounting for approximately 56% of the extractable arsenic with Cly-riboside (26%), PO_4-riboside (13%) and As(Ⅲ) (5%) also present. Under low As(Ⅴ):high PO_4~(3-) conditions As(Ⅴ) only accounted for approximately 18% of the extractable arsenic with Gly-riboside (44%) and PO_4-riboside (38%) more prominent Under low PO_4~(3-) conditions Gly-riboside was the major arsenic species (84-92%) irrespective of As(Ⅴ) exposure with As(Ⅴ) (8-10%) and PO_4-riboside (6%) also present This study demonstrates that D. tertiolecta alters its arsenic and nutrient cycling processes depending on the chemical composition of the culture media or surrounding environment Future research should endeavour to culture laboratory phytoplankton at nutrient and arsenic concentrations that mimic the natural environment to ensure that observations generated in laboratory experiments can be used to describe arsenic cycling in marine systems.
机译：As（Ⅴ）（50μgL ~~（-1）＆2）μgL〜（-1））和PO_4〜（3-）（3 mgL〜（-1）＆0.12 mg L〜（-1）的影响分别和组合研究了海洋浮游植物杜氏盐藻对砷循环的暴露。暴露于50μgL〜（-1）As（V）时，D螨组织中的总砷浓度为8至10μgg〜（-1），而暴露于2-4μgg〜（-1） 2μgL〜（-1）As（Ⅴ），P0_4〜（3-）暴露对总砷浓度几乎没有影响。在暴露于高As（Ⅴ）：高PO_4〜（3-）浓度的培养物中，砷在三个主要生化细胞组分（脂质，水，残留物）之间均匀分布（约33％）。在低As（Ⅴ）：高PO_4〜（3-）暴露下，砷主要是脂溶性的（40-55％），残留物结合（30-40％）。当暴露于高As（Ⅴ）：低PO_4〜（3-）浓度时，砷主要是残基结合（50-70％）和脂溶性（20-40％），而暴露于低As（Ⅴ）：低PO_4〜 （3-）暴露砷主要与残基结合（75-80％）。 As（Ⅴ）是在所有D. tertiolecta培养物中的水溶性细胞组分中存在的主要砷物质。在高PO_4〜（3-）浓度下，As（Ⅴ）占可萃取水溶性砷中的93％以上，而As（Ⅲ）是唯一检测到的其他物种。相反，在低PO_4〜（3-）浓度下，As（Ⅴ）占可萃取砷的66-71％，其中As（Ⅲ）（16-26％）和PO_4-核糖苷（8-13％）存在。在高As（Ⅴ）：高PO_4〜（3-）浓度下，As（Ⅴ）是水解脂质提取物中的主要砷物质，约占Cly-核苷（26％），PO_4-核苷（56％）的可提取砷的百分比。也存在13％）和砷（Ⅲ）（5％）。在低As（Ⅴ）：高PO_4〜（3-）条件下，As（Ⅴ）仅约占可提取砷的18％，其中Gly-核糖苷（44％）和PO_4-核糖苷（38％）更突出〜（3-）条件糖基核糖苷是主要的砷种类（84-92％），而与砷（Ⅴ）（8-10％）和PO_4-核糖苷（6％）接触的砷（Ⅴ）无关。证明D. tertiolecta会根据培养基或周围环境的化学成分改变其砷和养分循环过程可用于描述海洋系统中的砷循环。

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  • 来源
    《Marine Chemistry》 |2013年第20期|78-85|共8页
  • 作者

    Elliott G. Duncan; William A. Maher; Simon D. Foster; Frank Krikowa;

  • 作者单位

    Ecochemistiy Laboratory, Institute for Applied Ecology, University of Canberra, University Drive, Bruce, ACT 2601, Australia CSIRO Plant Industry, Centre for Environment and Life Sciences, Underwood Avenue, Floreat, WA 6014, Australia;

    Ecochemistiy Laboratory, Institute for Applied Ecology, University of Canberra, University Drive, Bruce, ACT 2601, Australia;

    Ecochemistiy Laboratory, Institute for Applied Ecology, University of Canberra, University Drive, Bruce, ACT 2601, Australia;

    Ecochemistiy Laboratory, Institute for Applied Ecology, University of Canberra, University Drive, Bruce, ACT 2601, Australia;

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  • 正文语种 eng
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  • 关键词

    Arsenic cycling; Nutrient cycling; Phosphate; Arsenate; Dunaliella tertiolecta; Marine phytoplankton; Batch phytoplankton cultures;

    机译：砷循环;营养循环;磷酸盐;砷酸盐杜氏杜氏藻;海洋浮游植物;浮游植物分批培养;

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