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首页> 外文期刊>Marine Chemistry >Advanced Characterization Of Marine Dissolved Organic Matter By Combining Reversed-phase Liquid Chromatography And Ft-icr-ms
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Advanced Characterization Of Marine Dissolved Organic Matter By Combining Reversed-phase Liquid Chromatography And Ft-icr-ms

机译：反相液相色谱与Ft-icr-ms结合对海洋溶解有机物的高级表征

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摘要

Marine dissolved organic matter (DOM) was separated by reversed-phase (RP) liquid chromatography method and analyzed with fluorescence/absorption detection and Fourier transform ion cyclotron mass spectrometry (FT-ICR-MS). The three key characteristics of the RP method are: (a) The C18-RP column chosen provides enhanced separation when the aqueous phase is 100% buffer-free water, and it does not degrade over time; (b) the water eluent adjusted to pH 7 significantly improves the resolution of water soluble compounds; (c) the initial flow maintained at low levels improves the separation of polar compounds. In samples, containing "fresh" DOM, specific peaks were detected, which were absent in "old" DOM samples. The combination with size exclusion chromatography (SEC) also demonstrated the relation between polarity and molecular size of DOM. FT-ICR-MS was applied to evaluate the quality of separation on a molecular scale demonstrating that physico-chemical characteristics of DOM can be related to molecular formulas. Sample extracts were separated into 4 preparative fractions, and a large suite of the identified molecular formulas only occurred in specific fractions. This is an important basis for the application of further analytical techniques in order to perform a more target-oriented analysis aiming at the determination of source and process biomarkers for DOM.
机译：通过反相（RP）液相色谱法分离海洋溶解的有机物（DOM），并通过荧光/吸收检测和傅立叶变换离子回旋加速器质谱（FT-ICR-MS）进行分析。 RP方法的三个主要特征是：（a）当水相为100％无缓冲水时，选择的C18-RP色谱柱可提供增强的分离效果，并且不会随时间而降解； （b）将水洗脱液的pH值调节至7，可显着提高水溶性化合物的分离度； （c）将初始流量保持在较低水平可改善极性化合物的分离。在包含“新鲜” DOM的样品中，检测到特定的峰，而在“旧” DOM样品中不存在。与尺寸排阻色谱法（SEC）的结合也证明了DOM的极性和分子大小之间的关系。 FT-ICR-MS用于评估分子质量分离的质量，证明DOM的理化特性可能与分子式有关。样品提取物被分为4个制备级分，一大批已鉴定的分子式仅在特定级分中出现。这是应用进一步的分析技术以执行更针对目标的分析的重要基础，旨在确定DOM的来源和过程生物标记。

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