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首页> 外文期刊>Marine Chemistry >Hydrolysis patterns and the production of peptide intermediates during protein degradation in marine systems
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Hydrolysis patterns and the production of peptide intermediates during protein degradation in marine systems

机译：海洋系统蛋白质降解过程中的水解模式和肽中间体的产生

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摘要

Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) was used to evaluate the degradation of the protein bovine serum albumin (BSA) added to seawater. The production of peptides during degradation, the size of the peptides produced and the within-protein locations of protease attack were all monitored in an effort to evaluate whether specific types of proteases or specific peptide bond locations were targeted during BSA degradation. Analysis of products from the bacterial degradation of proteins in both seawater and sediment pore water revealed the creation and release of peptide intermediates into the medium. Peptides observed in seawater degradation experiments were all less than 40 amino acids long, whereas sedimentary digestions of the same protein generated peptide fragments in the pore water ranging from 110 to 508 amino acids in length. Neither of the environments demonstrated recurring cleavages adjacent to one amino acid or functional group that might be indicative of the action of a single protease. Instead, it appears that a mixture of bacterial proteases were involved in the degradation of protein. The MALDI-TOF-MS method presented, in combination With these results, can help us delineate some of the processes responsible in the initial stages of degradation and better understand how proteins are partitioned between dissolved and solid phases in marine systems.
机译：使用基质辅助激光解吸/电离飞行时间质谱仪（MALDI-TOF-MS）评估添加到海水中的牛血清白蛋白（BSA）的降解。为了评估在BSA降解过程中是否靶向特定类型的蛋白酶或特定肽键位置，对降解过程中肽的产生，所产生肽的大小以及蛋白酶攻击的蛋白内位置均进行了监测。对海水和沉积物孔隙水中蛋白质的细菌降解产物的分析表明，肽中间体的产生和释放到培养基中。在海水降解实验中观察到的肽长都少于40个氨基酸，而相同蛋白质的沉淀消化在孔隙水中产生的肽片段长度在110至508个氨基酸之间。两种环境均未显示与一个氨基酸或官能团相邻的重复切割，这可能表明单一蛋白酶的作用。相反，似乎细菌蛋白酶的混合物参与了蛋白质的降解。结合这些结果，提出的MALDI-TOF-MS方法可以帮助我们描述降解初期的一些过程，并更好地了解蛋白质如何在海洋系统的溶解相和固相之间分配。

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