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首页> 外文期刊>Marine ecology progress series >Epibiotic bacterial community of Sphaeroma serratum (Crustacea, Isopoda): relationship with molt status
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Epibiotic bacterial community of Sphaeroma serratum (Crustacea, Isopoda): relationship with molt status

机译：锯缘肉芽肿（甲壳纲，等足目）的表观生物细菌群落：与蜕皮状态的关系

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摘要

Sphaeroma serratum is a marine isopod species that inhabits seashores from Europe to West Africa. The individuals live under stones in direct contact with reduced sediments and harbour a diverse bacterial community on the cuticle of their pleopods. We investigated the diversity of these epibiotic bacteria on male (pubescent and senescent) and female specimens with electron microscopic observations and molecular tools. The microbial community of S. serratum was shown to be composed of at least 5 bacterial morphotypes observed on the pleopodal cuticle in all male specimens. Using fluorescence in situ hybridization, we identified 5 major phylogenetic groups ( α-, β-, γ- and δ-Proteobacteria and Archaea) whereas denaturing gradient gel electro-phoresis (DGGE) of PCR-amplified 16S rRNA gene fragments of epibiotic bacteria revealed 50 bands. The bacterial community associated with S. serratum seems more diverse than in other marine crustaceans, such as Rimicaris. The relative diversity of this bacterial community was also studied in relation to the molt cycle. The comparison of DGGE band patterns of several individuals from female, pubescent male and senescent male groups revealed that the bacterial community diversity was dependent on the sex and the age of the individuals and more generally on the molt status.
机译：锯缘肉食蟹（Sphaeroma serratum）是一种海洋等足动物，栖息于从欧洲到西非的海滨。这些个体生活在与减少的沉积物直接接触的石头下面，并在其腹足表皮上拥有一个多样化的细菌群落。我们用电子显微镜观察和分子工具研究了雄性（青春期和衰老）和雌性标本上这些表生细菌的多样性。在所有雄性标本中，在腹足表皮上观察到的S. serratum微生物群落至少由5种细菌形态型组成。使用荧光原位杂交，我们确定了5个主要的系统发生组（α-，β-，γ-和δ-变形杆菌和古细菌），而PCR扩增的表观细菌的16S rRNA基因片段的变性梯度凝胶电泳（DGGE）显示50个乐队。与锯齿沙门氏菌相关的细菌群落似乎比其他海洋甲壳类动物（如Rimicaris）更多样化。还研究了该细菌群落相对于蜕皮周期的相对多样性。比较来自女性，青春期男性和衰老男性群体的几个个体的DGGE带模式，发现细菌群落多样性取决于个体的性别和年龄，并且更普遍地取决于蜕皮状态。

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  • 来源
    《Marine ecology progress series》 |2012年第21期|p.11-27|共17页
  • 作者

    Audrey Caro; Arthur Escalas; Corinne Bouvier; Evelyse Grousset; Nicole Lautredou-Audouy; Cecile Roques; Mireille Charmantier; Olivier Gros;

  • 作者单位

    UMR-CNRS 5119, Laboratoire Ecologie des Systemes Marins Cotiers, Universite Montpellier II, 34095 Montpellier, Cedex 5, France;

    UMR-CNRS 5119, Laboratoire Ecologie des Systemes Marins Cotiers, Universite Montpellier II, 34095 Montpellier, Cedex 5, France;

    UMR-CNRS 5119, Laboratoire Ecologie des Systemes Marins Cotiers, Universite Montpellier II, 34095 Montpellier, Cedex 5, France;

    UMR-CNRS 5119, Laboratoire Ecologie des Systemes Marins Cotiers, Universite Montpellier II, 34095 Montpellier, Cedex 5, France;

    INSERM U554, CNRS UMR 5048, Montpellier, France;

    UMR-CNRS 5119, Laboratoire Ecologie des Systemes Marins Cotiers, Universite Montpellier II, 34095 Montpellier, Cedex 5, France;

    UMR-CNRS 5119, Laboratoire Ecologie des Systemes Marins Cotiers, Universite Montpellier II, 34095 Montpellier, Cedex 5, France;

    UMR-CNRS 7138, Systematique-Adaptation-Evolution, Equipe 'Biologie de la mangrove', BP 592, Universite des Antilles et de la Guyane, 97159 Pointe-a-Pitre Cedex, Guadeloupe, France;

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  • 关键词

    sphaeroma; molt cycle; epibiotic biofilm; DGGE band pattern; FISH; crustacean;

    机译：血肿蜕皮周期表生生物膜DGGE带型;鱼;甲壳类;

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