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Seasonal and spatial influences on gene expression in Antarctic krill Euphausia superba

机译:季节和空间影响南极磷虾紫杉的基因表达。

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The high latitudes inhabited by Antarctic krill Euphausia superba Dana are characterised by extreme changes in seasonal conditions, with very low food availability, long periods of darkness and a massive expansion of sea ice in autumn and winter. How krill adapt to these conditions has yet to be determined, and it was the purpose of this study to gain more insight by investigating the differences between summer and winter krill populations at the molecular level. To achieve this, we used a cDNA microarray to compare gene expression between female adult krill caught during summer and winter near the Antarctic Peninsula (60° S), and between Peninsula krill and krill caught near South Georgia (54° S) during the same winter. The Peninsula seasonal comparison showed up-regulation in summer krill of genes involved in feeding and digestion, respiration, motor activity and immunity. Summer krill also showed evidence of increased vitelloge-nesis, with an insulin-related peptide up-regulated and neuroparsin down-regulated. The comparison between the different overwintering locations showed an up-regulation in the South Georgia krill of genes involved in feeding and digestion, and immunity. There was, however, no differential expression of genes involved in respiration, motor activity or vitellogenesis, suggesting that despite the above differences, both populations of overwintering krill were in a quiescent state. This study shows how krill physiology changes seasonally at the molecular level and how these changes are flexible according to the overwintering environment.
机译:南极磷虾Euphausia superba Dana所处的高纬度地区具有季节性条件的极端变化,食物供应量非常低,长期处于黑暗中,并且在秋冬季节海冰大量膨胀。磷虾如何适应这些条件尚未确定,本研究的目的是通过在分子水平上研究夏季和冬季磷虾种群之间的差异来获得更多的见识。为此,我们使用cDNA微阵列比较了南极半岛(60°S)夏季和冬季捕获的成年雌性磷虾,以及同一时期南乔治亚(54°S)附近捕获的雌性磷虾的基因表达。冬季。半岛季节比较显示,夏季磷虾中与进食和消化,呼吸,运动活动和免疫有关的基因上调。夏季磷虾还显示出玻璃体肌组织增多的证据,胰岛素相关肽被上调,而神经素则被下调。不同越冬地点之间的比较显示,南乔治亚磷虾的上调涉及进食,消化和免疫力。但是,与呼吸,运动活动或卵黄发生有关的基因没有差异表达,这表明尽管存在上述差异,但两个越冬磷虾种群都处于静止状态。这项研究显示了磷虾生理学如何在分子水平上季节性变化以及这些变化如何根据越冬环境而灵活变化。

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