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首页> 外文期刊>Marine ecology progress series >Kinetics of alkaline phosphatase activity, and effect of phosphate enrichment: a case study in the NW African upwelling region
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Kinetics of alkaline phosphatase activity, and effect of phosphate enrichment: a case study in the NW African upwelling region

机译：碱性磷酸酶活性的动力学，以及磷酸盐富集的影响：以西北非洲上升流地区为例

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Alkaline phosphatase (AP) kinetic experiments were performed in a broad range of trophic conditions in the transition zone between the North African upwelling and the open ocean, in order to investigate the effect of nutrient richness on kinetic parameters. Turnover times decreased from 224 h in the open ocean to 13 h in the upwelling region. K + S_n showed the lowest values at the most eutrophic station (27 nM), while at this station V_m reached its maximum value of 2.1 nM h~(-1). Nevertheless, as multiphasic kinetics occurred, kinetic parameter values depended largely on the fitting technique used. Response of AP activity (APA) to phosphate enrichment was also studied along a trophic gradient at the surface and the chlorophyll maximum level. Inhibition of APA reached 50 % with only 0.1 μM of phosphate added, and rose to 80 to 96% inhibition by additions of 1μM at the oligotrophic stations, whereas in the most eutrophic station, phosphate amendments had no effect. Inhibition was higher at the surface and towards oligotrophy. The lack of inhibition in the upwelling station and the low K + S_n encountered suggest that the APA of eutrophic waters was mainly from bacterial origin. This supports the hypothesis of APA being mainly involved in supplying easily assimilable organic carbon to bacteria in eutrophic waters.
机译：为了研究营养丰富度对动力学参数的影响，在北非上升流与公海之间的过渡带中，在广泛的营养条件下进行了碱性磷酸酶（AP）动力学实验。周转时间从公海的224小时减少到上升流地区的13小时。 K + S_n在最富营养化站（27 nM）处显示最低值，而在该站V_m达到其最大值2.1 nM h〜（-1）。然而，由于发生了多相动力学，动力学参数值很大程度上取决于所使用的拟合技术。还沿着表面的营养梯度和叶绿素最大水平研究了AP活性（APA）对磷酸盐富集的响应。仅添加0.1μM磷酸盐，对APA的抑制作用达到50％，而在贫营养站，通过添加1μM，对APA的抑制作用上升至80％至96％，而在最富营养的站，磷酸盐修饰物无效。表面和向寡营养的抑制作用更高。在上升流站缺乏抑制作用，并且遇到的K + S_n低表明富营养化水域的APA主要来自细菌。这支持了APA的假设，即APA主要涉及向富营养化水中的细菌提供易于吸收的有机碳。

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