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首页> 外文期刊>Marine ecology progress series >Regulation of growth and energetics of a marine bacterium by nitrogen source and iron availability
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Regulation of growth and energetics of a marine bacterium by nitrogen source and iron availability

机译：通过氮源和铁的利用来调节海洋细菌的生长和能量

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This study examined the effect of nitrogen source and iron availability on growth rates, electron transport system (ETS) activity and growth efficiency for the marine gamma-proteobacterium Vibrio harveyi. We found that all 3 parameters increased with increasing iron concentration and varied as a function of nitrogen source (amino acids, ammonium, and nitrate). At low iron concentrations, growth rates were highest and lowest in the amino acid and nitrate media, respectively. Rates were similar for the amino acid and the ammonium plus glucose media with sufficient iron (>20 nM). Growth rates were lowest when nitrate was the nitrogen source, regardless of iron concentration. ETS activity per cell in the different media mostly followed the pattern in growth rates, although ETS activity for cells using nitrate was even lower than would have been predicted from the growth rate data. Growth efficiencies for iron-sufficient cells did not vary substantially among cells using the 3 nitrogen sources (45 to 55%), but they were substantially higher than growth efficiencies of iron-depleted cells (<10%). In media without added iron, cells grown on nitrate had the lowest growth efficiency, >2-fold lower than iron-stressed cells using ammonium or amino acids as nitrogen sources. Although results from any single cultured bacterium should be applied cautiously to natural microbial assemblages, our data may help explain the low growth efficiencies and growth rates in iron-limited oceanic regimes with high nitrate but low chlorophyll concentrations.
机译：这项研究检查了氮源和铁的可用性对海洋γ-变形杆菌哈维氏弧菌的生长速率，电子传输系统（ETS）活性和生长效率的影响。我们发现，所有三个参数都随铁浓度的增加而增加，并且随氮源（氨基酸，铵和硝酸盐）的变化而变化。在低铁浓度下，氨基酸和硝酸盐培养基的生长速率分别最高和最低。氨基酸，铵盐和葡萄糖介质中铁含量充足（> 20 nM）的速率相似。当硝酸盐作为氮源时，无论铁的浓度如何，生长速率最低。尽管使用硝酸盐的细胞的ETS活性甚至比根据增长率数据预测的更低，但在不同培养基中每个细胞的ETS活性大多遵循增长率的模式。在使用3种氮源的细胞中，铁充足的细胞的生长效率没有实质性的变化（45％至55％），但它们显着高于贫铁细胞的生长效率（<10％）。在不添加铁的培养基中，在硝酸盐上生长的细胞的生长效率最低，比使用铵或氨基酸作为氮源的铁胁迫细胞低2倍以上。尽管应谨慎地将任何一种培养细菌的结果应用于天然微生物组合，但我们的数据可能有助于解释在硝酸盐含量高但叶绿素浓度低的铁限制海洋条件下低的生长效率和增长率。

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