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首页> 外文期刊>Marine ecology progress series >Molecular identification of the diet of Sardina pilchardus larvae in the SW Mediterranean Sea
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Molecular identification of the diet of Sardina pilchardus larvae in the SW Mediterranean Sea

机译：地中海西南沙丁鱼沙丁鱼幼虫饮食的分子鉴定

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摘要

Molecular techniques provide new insights into the feeding strategies and diets of planktonic organisms such as the larvae of marine fish. We applied multiplex PCR to obtain the first estimates of the diets of larval European sardine Sardina pilchardus in the Alboran Sea (SW Mediterranean), where this species represents an important fishery resource. The feasibility of this technique was tested in a 26 h continuous survey of a shoal of larvae (10.80 +/- 0.73 mm standard length, mean +/- SD). Multiplex PCR was designed to detect the presence of 5 copepod species, a microplanktonic dinoflagellate (Gymnodinium) and the picoeukaryote algae family Prasinophyceae in larval guts. We simultaneously sampled sardine larvae and their potential prey (pico- to mesoplankton) and compared diel variability of the prey field and ingested items. Microplankton was dominated by flagellates, and copepods represented the most abundant mesozooplankton, reaching peak abundance at night. Prey DNA was detected throughout the entire diel cycle, despite no visible prey in the guts of larvae collected at night. Sardine larvae preyed on early life stages of the most abundant copepod species (Oncaea waldemari, Paracalanus indicus and Temora stylifera), suggesting an opportunistic foraging behaviour. The use of multiplex PCR allowed species-level identification of ingested nauplii and protists, which otherwise would remain unidentified.
机译：分子技术为浮游生物（例如海鱼幼虫）的摄食策略和饮食提供了新的见识。我们应用多重PCR来获得阿尔伯海（西南地中海）幼体欧洲沙丁鱼沙丁鱼（Sardina pilchardus）的饮食的初步估计，该物种是重要的渔业资源。在连续26小时的幼虫浅滩调查中测试了该技术的可行性（标准长度为10.80 +/- 0.73 mm，平均+/- SD）。多重PCR的目的是检测幼虫内脏中5种species足类，微浮游生物鞭毛藻（Gymnodinium）和小核藻类藻类科。我们同时采样了沙丁鱼幼虫及其潜在的猎物（从皮鱼到中浮游生物），并比较了猎物场和被摄食物的diel变异性。浮游生物主要由鞭毛虫组成，pe足类动物是中浮游动物中最丰富的，在夜间达到高峰。尽管在夜间收集的幼虫胆汁中没有可见的猎物，但在整个diel周期中都检测到了猎物DNA。沙丁鱼幼虫捕食了最丰富的co足类物种（Oncaea waldemari，Paracalanus indicus和Temora stylifera）的早期生命，这表明它是一种机会性的觅食行为。多重PCR的使用允许对摄入的无节幼体和原生生物进行物种级鉴定，否则将无法鉴定。

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