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首页> 外文期刊>Marine ecology >Efficiency of calcein tagging on juveniles of the sea urchins Diademaafricanum and Paracentrotuslividus
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Efficiency of calcein tagging on juveniles of the sea urchins Diademaafricanum and Paracentrotuslividus

机译：钙黄绿素标记在海胆幼体Diademaafricanum和Paracentrotuslividus上的效率

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摘要

We evaluated the effectiveness of chemical tagging with the fluorescent marker calcein for two key species of herbivorous sea urchins, Diademaafricanum and Paracentrotuslividus, to facilitate medium- and long-term ecological experiments. In total, 98 juveniles of D.africanum and 98 P.lividus were tagged with this fluorescent marker, with 12 combinations of different tagging techniques (chemical bath or injection), concentrations of calcein (2, 10 and 20mg·l−1), and soaking times (2, 4 and 24h). Respective control treatments were conducted by means of seawater injection and bathing. The success of tagging was assessed after a month of feeding individuals adlibitum with the algae Dictyota sp. Sea urchins were dissected and their Aristotle's lanterns cleaned with 10% sodium hypochlorite to examine these structures under UV light using a binocular microscope. Each species was evaluated in terms of survival, percentage of tagged individuals and intensity of the resulting tag. The results showed that the method of soaking individuals of both species for 24h in concentrations of calcein of 10 and 20mg·l−1 gave the highest percentage survival (100%) and the resulting tags were clearly visible.
机译：我们评估了用荧光标记钙黄绿素进行化学标记对草食性海胆这两个关键物种DIADEMAAFRICANUM和Paracentrotuslividus的有效性，以促进中长期生态实验。总共，用该荧光标记物标记了98个非洲D.africanum和98.lividus少年，并结合了12种不同标记技术（化学浴或注射），钙黄绿素浓度（2、10和20mg·l-1），和浸泡时间（2、4和24小时）。通过海水注入和沐浴进行相应的对照处理。一个月后，给个体随意喂食藻类Dictyota sp，评估标记的成功。解剖海胆，用10％次氯酸钠清洗其亚里斯多德的灯笼，以双目显微镜在紫外线下检查这些结构。根据存活率，标记个体的百分比和所得标记的强度评估每种物种。结果表明，在10和20mg·l-1的钙黄绿素浓度下将两种物种的个体浸泡24h的方法具有最高的存活百分比（100％），并且所产生的标签清晰可见。

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