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首页> 外文期刊>Marine Environmental Research >Effects of different nitrate and phosphate concentrations on the growth and toxin production of an Alexandrium tamarense strain collected from Drake Passage
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Effects of different nitrate and phosphate concentrations on the growth and toxin production of an Alexandrium tamarense strain collected from Drake Passage

机译：不同硝酸盐和磷酸盐浓度对从德雷克海峡采集的亚历山大番茄的生长和毒素产生的影响

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摘要

Nitrate (N) and phosphate (P) are believed to be two of the most important nutrients for the growth and toxin production for Alexandrium species. The study of the growth and toxicity characteristics of the Alexandrium spp. under the change of N and P can help us to understand the dynamics of algal bloom and toxification events in natural environments. A strain of Alexandrium tamarense (designated as Kci) was successfully isolated from the Drake Passage in 2001 and the clonal culture has been kept in our laboratory (Ho et al., 2003,2012). In order to extend our understanding on the growth physiology and toxicity of this A. tamarense strain, growth and cellular toxin content were examined in unialgal batch cultures under different concentrations of N and P. The effects of variable N, P concentrations on growth, cellular toxicity (fg STXeq. cell~(-1)), and toxin composition (% molar) were determined in both exponential and stationary growth phases. The toxin profile, determined by high-performance liquid chromatography with fluorescence detection (HPLC-FD), was found to be remained relatively stable and was consistently dominated by the N-sulfocarbamoyl C-toxins (>90%) under different conditions and growth phases. There were also trace amounts of other carbamate gonyautoxins consistently expressed. The cellular toxicity varied under different N and P concentrations, as well as different growth stages. A positive correlation was observed between cellular toxicity and N concentrations, but the toxicity was enhanced when P was depleted. Both cell densities and growth rate of the cells were severely suppressed under N-or P-depletion. However, the biovolume of the cells tended to be larger at N- or P-depleted cultures. Results from the present study provide valuable insight for the ecophysiology of Alexandrium species in the coastal ecosystem of Drake Passage.
机译：硝酸盐（N）和磷酸盐（P）被认为是亚历山大藻生长和毒素产生的两种最重要的营养素。亚历山大藻的生长和毒性特性的研究。在氮和磷的变化下，可以帮助我们了解自然环境中藻华和毒化事件的动态。 2001年，从德雷克通道成功分离出一株亚历山大蜜柳（Alexandium tamarense）（命名为Kci），克隆培养物已保存在我们的实验室中（Ho等，2003，2012）。为了扩展我们对这种番茄的生长生理和毒性的了解，在不同氮和磷浓度的单藻分批培养物中检测了生长和细胞毒素含量。可变的氮，磷浓度对细胞生长，细胞生长的影响测定了指数生长期和固定生长期的毒性（f STXeq。cell〜（-1））和毒素组成（摩尔％）。通过高效液相色谱和荧光检测（HPLC-FD）确定的毒素分布被发现保持相对稳定，并在不同条件和生长阶段始终被N-磺氨基甲酰基C毒素（> 90％）所支配。 。也有痕量的其他氨基甲酸酯型淋菌毒素持续表达。在不同的氮和磷浓度以及不同的生长阶段下，细胞毒性都不同。观察到细胞毒性与N浓度呈正相关，但是当P耗尽时，毒性增强。在N-或P-耗尽下，细胞密度和细胞生长速率均被严重抑制。但是，在缺N或P的培养物中，细胞的生物量往往会更大。本研究的结果为德雷克海峡沿海生态系统中亚历山大藻物种的生态生理学提供了宝贵的见识。

著录项

  • 来源
    《Marine Environmental Research》 |2012年第10期|p.62-69|共8页
  • 作者

    Thomas Chun-Hung Lee; Oi-Ting Kwok; Kin-Chung Ho; Fred Wang-Fat Lee;

  • 作者单位

    School of Science and Technology, The Open University of Hong Kong, Hong Kong, China;

    School of Science and Technology, The Open University of Hong Kong, Hong Kong, China;

    School of Science and Technology, The Open University of Hong Kong, Hong Kong, China;

    School of Science and Technology, The Open University of Hong Kong, Hong Kong, China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    alexandrium; drake passage; growth; nitrogen; phosphate; toxicity;

    机译：亚历山大草;德雷克通道增长氮;磷酸盐毒性;

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