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首页> 外文期刊>Marine Environmental Research >Raman microspectrometry as a powerful tool for a quick screening of thiotrophy: An application on mangrove swamp meiofauna of Guadeloupe (F.W.I.)
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Raman microspectrometry as a powerful tool for a quick screening of thiotrophy: An application on mangrove swamp meiofauna of Guadeloupe (F.W.I.)

机译：拉曼光谱法作为快速筛选硫营养素的有力工具：在瓜德罗普岛（F.W.I.）的红树林沼泽藻类动物中的应用

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摘要

The mangrove swamp environment constitutes a sulphide rich habitat harbouring some thioautotrophic organisms. The ciliate Zoothamnium niveum and the nematode Eubostrichus dianae, both known to live associated with bacterial sulphide-oxidizing ectosymbionts, were analysed as positive controls by Raman microspectrometry. The detection of the 3 Raman bands characteristic of elemental sulphur (S_8) allows us to define a positive model of sulphide-oxidizing symbiotic invertebrates and by extrapolation, of thioautotrophic organisms. A fast screening using this tool was carried out on eukaryotic organisms such as hydrozoan, nematodes, annelids, copepods, and ciliate {Pseudovorticella sp.) and on free-living filamentous bacteria found on decomposing leaves in order to detect thioautotrophic organisms. The Raman microspectrometry permits us: (i) to reveal thioautotrophic metabolism of free-living bacteria (Beggiatoa sp.) and even for Archaea and (ii) to detect sulphide-oxidizing endosymbiotic and ectosymbiotic bacteria associated with the Bivalve Lucina pectinata and Pseudovorticella sp., respectively. Raman microspectrometry represents a fast, easy and non destructive technique which can be used on living organisms without constraints of sample size. The Raman analysis can also be completed by ultra-structural analysis (SEM, TEM) on the same sample.
机译：红树林的沼泽环境构成了富含硫代自养生物的富含硫化物的栖息地。通过拉曼光谱法分析了已知与细菌硫化物-氧化胞外共生体相关的纤毛的梭菌梭状芽胞杆菌（Zoothamnium niveum）和线虫（Eubostrichus dianae）作为阳性对照。对元素硫（S_8）的3个拉曼谱带特征的检测使我们能够定义一个硫化物氧化共生无脊椎动物和硫代自养生物的外推正模型。为了检测硫代自养生物，对真核生物如水生动物，线虫，线虫，co足类和纤毛虫（假单胞菌）进行了快速筛选，并对分解叶片上发现的自由生存的丝状细菌进行了筛选。拉曼光谱法使我们能够：（i）揭示自由生存细菌（Beggiatoa sp。）甚至古细菌的硫代自养代谢，以及（ii）检测与双歧杆菌Lucina pectinata和Pseudovorticella sp相关的硫化物氧化内共生和外共生细菌。 ， 分别。拉曼光谱法是一种快速，简便且无损的技术，可用于活生物体而不受样品量的限制。拉曼分析还可以通过对同一样品进行超结构分析（SEM，TEM）来完成。

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  • 来源
    《Marine Environmental Research》 |2010年第5期|p.382-389|共8页
  • 作者

    Leslie C. Maurin; David Himmel; Jean-Louis Mansot; Olivier Gros;

  • 作者单位

    UMR-CNRS 7138, Systematique-Adaptation-Evolution, Equipe «Biologie de la mangrove», Universite des Antilles et de la Guyane, UFR des Sciences Exactes et Naturelles,Departement de Biologie, B.P. 592, 97159 Pointe-a-Pitre Cedex, Guadeloupe, France;

    CTSl - Universite des Antilles et de la Cuyane, UFR des sciences exactes et naturelles, Departement de Physique, B.P. 592, 97159 Pointe-a-Pitre Cedex, Guadeloupe, France;

    CTSl - Universite des Antilles et de la Cuyane, UFR des sciences exactes et naturelles, Departement de Physique, B.P. 592, 97159 Pointe-a-Pitre Cedex, Guadeloupe, France;

    UMR-CNRS 7138, Systematique-Adaptation-Evolution, Equipe «Biologie de la mangrove», Universite des Antilles et de la Guyane, UFR des Sciences Exactes et Naturelles,Departement de Biologie, B.P. 592, 97159 Pointe-a-Pitre Cedex, Guadeloupe, France;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    raman microspectrometry; sulphide-oxidizing symbiosis; thiotrophic organisms; mangrove; meiofauna;

    机译：拉曼光谱法;硫化物共生;硫营养生物;红树林;淡水鱼;

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