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Molecular methods for cost-efficient monitoring of HAB (harmful algal bloom) dinoflagellate resting cysts

机译:用于经济高效地监测有害藻华(HAB)的鞭毛安息囊的分子方法

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摘要

Cyst abundance and identity are essential for understanding and predicting blooms, and for assessing the dispersal of toxic target dinoflagellate species by natural or human mediated ways, as with ballast waters. The aim of this study was to apply rapid, specific and sensitive qPCR assays to enumerate toxic dinoflagellate cysts in sediment samples collected from Adriatic harbours. The molecular standard curves of various target species allowed obtaining the rDNA copy number per cyst. The analytical sensitivity for specific standard curves was determined to be 2 or 10 rDNA copies per reaction. The abundance varied in the range of 1-747 dinoflagellate cysts g(-1) dry weight. The assays showed greater sensitivity as compared to counts by light microscopy. This qPCR method revealed a powerful tool for the quantification of cysts from toxic dinoflagellate resting stages in sediment samples from Adriatic ports.
机译:囊肿的丰度和特性对于理解和预测水华,以及通过自然或人为介导的方法(如压舱水)评估有毒目标鞭毛鞭毛藻物种的扩散至关重要。本研究的目的是应用快速,特异性和灵敏的qPCR分析方法,对从亚得里亚海港口收集的沉积物样品中的有毒鞭毛藻囊进行计数。各种目标物种的分子标准曲线允许获得每个囊肿的rDNA拷贝数。每个反应的特定标准曲线的分析灵敏度确定为2或10个rDNA拷贝。丰度在1-747鞭毛囊肿g(-1)干重范围内变化。与通过光学显微镜的计数相比,该测定显示出更高的灵敏度。这种qPCR方法揭示了一种强大的工具,可用于量化来自亚得里亚海港口沉积物样品中有毒鞭毛藻休息阶段的囊肿。

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