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Electrowetting-on-dielectric chip with integrated screen-printed electrochemical sensor for rapid chemical analysis

机译：集成丝网印刷电化学传感器的介电电芯片，用于快速化学分析

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摘要

In this work, a screen-printed electrochemical detector was fabricated on a digital electrowetting-on-dielectric (EWOD) microfluidic chip for the first time and employed for rapid chemical analysis. The digital microfluidic system consisted of a T-junction EWOD platform for merging buffer and analyte droplets and an electrochemical detector at the end of T-junction. The detector comprised three electrodes including carbon working, carbon counter and silver/silver chloride reference electrodes, The system was successfully fabricated by micro-fabrication and screen-printing processes and tested for electrochemical analyses of potassium hexacyanoferrate and hydrogen peroxide. The analyte and buffer droplets were controlled to mix with different concentrations and detected at the electrochemical detector using cyclic voltammetry. The results showed good detection performances with medium sensitivity, moderate detection limits, high stability and good reproducibility. In addition, the total analysis time including droplet mixing and CV measurement was shorter than 14 s. Therefore, the EWOD chip with integrated screen-printed carbon electrodes was a promising candidate for rapid automated chemical analysis.
机译：在这项工作中，第一次在数字电介质上电润湿（EWOD）微流控芯片上制造了丝网印刷的电化学检测器，并将其用于快速化学分析。数字微流体系统由一个用于合并缓冲液和分析物液滴的T型结EWOD平台和一个位于T型结末端的电化学检测器组成。该检测器包括三个电极，包括碳工作电极，碳对电极和银/氯化银参比电极。该系统通过微细加工和丝网印刷工艺成功制造，并进行了六氰合铁酸钾和过氧化氢的电化学分析测试。控制分析物和缓冲液液滴以不同浓度混合，并使用循环伏安法在电化学检测器上检测。结果表明检测性能良好，灵敏度中等，检测限适中，稳定性高，重现性好。此外，包括液滴混合和CV测量在内的总分析时间短于14 s。因此，具有集成丝网印刷碳电极的EWOD芯片是快速自动化化学分析的有希望的候选者。

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