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首页> 外文期刊>Materials Science and Engineering >Optimization, in-vitro release and in-vivo evaluation of bismuth-hyaluronic acid-melittin-chitosan modified with oleic acid nanoparticles computed imaging-guided radiotherapy of cancer tumor in eye cells
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Optimization, in-vitro release and in-vivo evaluation of bismuth-hyaluronic acid-melittin-chitosan modified with oleic acid nanoparticles computed imaging-guided radiotherapy of cancer tumor in eye cells

机译：用油酸纳米粒子改性铋 - 透明质酸 - 氯霉素 - 壳聚糖的优化，体外释放和体内评价在眼球细胞中癌症肿瘤的显影引导放射治疗

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摘要

Bismuth (Bi) has the ability to increase radiation and reduce the radiation dose. Conversely, HA plays an important role in healing damaged cells. The objective of this study was to synthesize bismuth-hyaluronic acid-melittin-chitosan modified with oleic acid nanoparticles (Bi@HA/MEL/CS-OA NPs), in order to treat cancerous eye cells with radiotherapy. In this study, the sol-gel method served to create these nanoparticles. Due to the optimization that was done, we were able to obtain the optimal number of variables to make these nanoparticles. Scanning electron microscopy (SEM), ultraviolet-visible spectrophotometry (UV-Vis), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), Radiological test (RT), MTT in vitro, in vivo were implemented in this research. The Taguchi test was used to optimize the parameters. The optimum conditions for melittin (MEL) loading in nanoparticles in the Taguchi test and experimental methods were 69.41% and 73.48%, respectively. The average size of the synthesized nanoparticles was between 10 and 20 nm. The shielding rate of synthesized NPs incorporated a thickness of 0.043 mm and at 50 kVp was 90.5%, which is comparable to lead which has a thickness of 0.087 mm. At a thickness of 0.06 mm, the shielding rate of bismuth was 98.7% and this was equivalent to 0.482 pdl. The release of MEL loading from the design process at a temperature of 37 °C and specific time in pH 7.2, amounted to 73.48%. To evaluate the toxicity of nanoparticles to the process design, 3-(4, 5-dimethyl (thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was performed using MCF-7 cancer cells. In vivo test confirmed the efficacy of the nanoparticles. Coating the Bi nanoparticles with CS helped to extend the half-life of the nanoparticles and prevent their adhesion.
机译：铋（BI）具有增加辐射并减少辐射剂量的能力。相反，HA在愈合受损细胞中发挥着重要作用。本研究的目的是合成用油酸纳米粒子（Bi @ HA / MEL / CS-OA NPS）改性的铋 - 透明质酸 - 氨基甲酸壳多糖，以治疗癌细胞的放射治疗。在该研究中，溶胶 - 凝胶方法用于产生这些纳米颗粒。由于完成的优化，我们能够获得最佳变量数来制作这些纳米颗粒。实施扫描电子显微镜（SEM），紫外 - 可见分光光度法（UV-VI），X射线衍射（XRD），傅里叶变换红外光谱（FT-IR），体内体内放射性试验（RT），MTT，MTT这项研究。 TAGUCHI测试用于优化参数。塔布奇试验中纳米颗粒中的甜瓜（Mel）加载的最佳条件分别为69.41％和73.48％。合成纳米颗粒的平均尺寸为10至20nm。合成NP的屏蔽速率掺入0.043mm和50kVP的厚度为90.5％，其与厚度为0.087mm的铅相当。厚度为0.06mm，铋的屏蔽速率为98.7％，这相当于0.482pdl。将MEL加载在37°C的温度和pH 7.2中的特定时间的温度释放至73.48％。为了评估纳米颗粒的毒性，使用MCF-7癌细胞进行3-（4,5-二甲基（噻唑-2-基）-2,5-二苯基四唑溴（MTT）测定。在体内测试中确认纳米颗粒的功效。用CS涂覆Bi纳米颗粒，有助于延长纳米颗粒的半衰期并防止它们的粘附性。

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