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首页> 外文期刊>Materialwissenschaft und Werkstofftechnik >The Influence of Lipid Dipole Moment and Interfacial Water Structure on Protein Adsorption to Mixed Lipid Monolayers
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The Influence of Lipid Dipole Moment and Interfacial Water Structure on Protein Adsorption to Mixed Lipid Monolayers

机译：脂质偶极矩和界面水结构对混合脂质单层蛋白质吸附的影响

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Protein interactions with a heterogeneous surface, such as a two-component lipid film, often deviate from simple additivity of the adsorption to the respective single component, "homogenous" films. This behavior was highlighted in previous research where adsorption of the negatively charged protein ferritin to mixed ca-tionic / non-ionic lipid films was greater than adsorption to either of the single component lipid films [Britt et al., Phys. Chem. Chem. Phys., 2, 4594-4599, 2000]. Here we investigate mixing induced changes in lipid dipole moments and local water structure as factors leading to amplification of monolayer charge and subsequent enhanced protein adsorption to the mixed lipid films. Surface potential and Fourier transform infrared spectroscopy (FTIR) revealed a reduction in both lipid tilt angle and gauche defects in the mixtures that would lead to a greater contribution of lipid alkyl-tail dipoles to the measured (normal) surface potential; however, as the orientation of the alkyl-tail dipole is from water (negative) to air (positive) the greater dipolar contribution for reduced tilt angles actually opposes adsorption of a negatively charged protein from the subphase. FTIR also revealed a mixing induced disordering of water solvating the cationic lipid head group, suggesting water dipoles were less effective in screening charge, which in turn would enhance the electrostatic interactions between anionic protein and the mixed cationic / nonionic lipid film.
机译：蛋白质与异质表面（例如两组分脂质膜）的相互作用通常偏离吸附对相应的单组分“均质”膜的简单加和。这种行为在以前的研究中得到了强调，其中带负电荷的蛋白质铁蛋白对混合阳离子/非离子脂质膜的吸附大于对任一单组分脂质膜的吸附[Britt et al。，Phys。化学化学Phys。，2，4594-4599，2000]。在这里，我们研究混合诱导的脂质偶极矩和局部水结构的变化，作为导致单层电荷放大和随后增强的蛋白质吸附到混合脂质膜的因素。表面电势和傅立叶变换红外光谱（FTIR）显示混合物中脂质倾斜角和gauche缺陷的减少，这将导致脂质烷基尾偶极子对测得的（正常）表面电势有更大的贡献。但是，由于烷基尾偶极子的方向是从水（负）到空气（正），因此更大的偶极对减小倾斜角的贡献实际上会阻止子相吸附带负电荷的蛋白质。 FTIR还揭示了混合诱导的水紊乱使阳离子脂质头基团溶解，表明水偶极子在筛选电荷方面效果较差，这反过来会增强阴离子蛋白与混合的阳离子/非离子脂质膜之间的静电相互作用。

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