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首页> 外文期刊>Materials science & engineering >In-vitro comparison of hydroxyapatite coatings obtained by cold spray and conventional thermal spray technologies
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In-vitro comparison of hydroxyapatite coatings obtained by cold spray and conventional thermal spray technologies

机译：通过冷喷涂和常规热喷涂技术获得的羟基磷灰石涂层的体外比较

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Hydroxyapatite (HA) coatings onto Ti6Al4V alloy substrates were obtained by several thermal spray technologies: atmospheric plasma spray (APS) and high velocity oxy fuel (HVOF), together with the cold spray (CS) technique. A characterization study has been performed by means of surface and microstructure analyses, as well as biological performance. In-vitro tests were performed with primary human osteoblasts at 1, 7 and 14 days of cell culture on substrates. Cell viability was tested by MTS and LIVE/DEAD assays, cell differentiation by alkaline phosphatase (ALP) quantification, and cell morphology was analyzed by scanning electron microscopy.The HA coatings showed an increase of HA crystallinity from 62,4% to 89%, but also an increase of hydrophilicity from similar to 32 degrees to 0 degrees, with the decrease of the operating temperature of the thermal spray techniques (APS > HVOF > CS). Additionally, APS HA coatings showed more surface micro-features than HVOF and CS HA coatings; cells onto APS HA coatings showed faster attachment by acquiring osteoblastic morphology in comparison with the rounded cell morphology observed onto CS HA coatings at 1 day of cell culture. HVOF HA coatings also showed proper cell adherence but did not show extended filopodia as cells onto APS HA coatings. However, at 14 days of cell culture, higher cell proliferation and differentiation was detected on HA coatings with higher crystallinity (HVOF and CS techniques). Cell attachment is suggested to be favoured by surface micro-features but also moderate surface wettability whereas cell proliferation and differentiation is suggested to be highly influenced by HA crystallinity and crystal size.
机译：Ti6Al4V合金基材上的羟基磷灰石（HA）涂层是通过几种热喷涂技术获得的：常压等离子体喷涂（APS）和高速氧燃料（HVOF），以及冷喷涂（CS）技术。已经通过表面和微观结构分析以及生物学性能进行了表征研究。在基质上进行细胞培养的第1、7和14天，对原代人成骨细胞进行了体外测试。通过MTS和LIVE / DEAD分析测试细胞活力，通过碱性磷酸酶（ALP）定量分析细胞分化，并通过扫描电子显微镜分析细胞形态.HA涂层显示HA结晶度从62.4％增加到89％，而且随着热喷涂技术操作温度的降低（APS> HVOF> CS），亲水性也从相似的32度增加到0度。此外，APS HA涂层比HVOF和CS HA涂层具有更多的表面微观特征。与在细胞培养1天时在CS HA涂层上观察到的圆形细胞形态相比，通过捕获成骨细胞形态，APS HA涂层上的细胞显示出更快的附着。 HVOF HA涂层还表现出适当的细胞粘附性，但在APS HA涂层上未显示出延长的丝状伪足。但是，在细胞培养14天后，在具有较高结晶度的HA涂层（HVOF和CS技术）上检测到较高的细胞增殖和分化。建议细胞附着有利于表面微观特征，但也适度的表面润湿性，而细胞增殖和分化则受HA结晶度和晶体尺寸的高度影响。

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