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Injectable and thermosensitive nanofibrous hydrogel for bone tissue engineering

机译：用于骨组织工程的可注射和热敏性纳米纤维水凝胶

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摘要

The use of injectable hydrogels is currently restricted by the challenge of achieving fast gelation, good mechanical strength, and cytocompatibility. Polymeric self-assembly is a potent tool for generating functional materials that combine multiple characteristics and can react to external factors. In this study, we have developed fiber-reinforced composite hydrogels that exhibits significantly enhanced mechanical strength, reduced gelling time, and excellent cytocompatibility. The practicability of developing a chitosan-based thermogelling solution using hydroxyapatite and polyelectrolyte complex (PEC) self-assembled fibers were evaluated. The effect of beta GP concentration on gelation time was studied by varying the concentration of beta GP added to the chitosan solution. Various combinations were tested to create a suitable hydrogel environment for cell encapsulation, growth, and proliferation at physiological pH and temperature. Determination of Young modulus revealed that PEC fibers reinforced hydrogel was three times stiffer than chitosan-beta GP gels. The gelation time was reduced to 3 min, and the hydrogels had porous structures and gels at physiological pH, temperature, and showed > 80% viability for MTT assay to MG63 cells. Moreover, confocal imaging of PEC fiber reinforced hydrogels showed noticeable viability and proliferation. The molecular interactions between gelling agents, polyelectrolytes, and hydroxyapatite were studied using FTIR. We investigated interfacial bonding between PEC fibers with beta GP, NaHCO3, and HAp. The combination of hydroxyapatite and polymer self-assembly technique improved the efficiency of injectable hydrogels that are helpful in minimally invasive applications.
机译：目前，可注射水凝胶的使用受到快速凝胶化，良好的机械强度和细胞相容性的挑战的限制。聚合物自组装是一种强大的工具，可用于生成功能材料，这些功能材料具有多种特性并可对外部因素做出反应。在这项研究中，我们开发了纤维增强的复合水凝胶，该水凝胶显示出显着增强的机械强度，减少的胶凝时间和出色的细胞相容性。评估了使用羟基磷灰石和聚电解质复合物（PEC）自组装纤维开发基于壳聚糖的热凝胶溶液的实用性。通过改变添加到壳聚糖溶液中的βGP的浓度研究了βgp浓度对胶凝时间的影响。测试了各种组合以创建合适的水凝胶环境，以在生理pH和温度下进行细胞包封，生长和增殖。杨氏模量的测定表明，PEC纤维增强的水凝胶比壳聚糖-βGP凝胶坚硬三倍。凝胶化时间减少至3分钟，并且水凝胶在生理pH，温度下具有多孔结构和凝胶，并且对MG63细胞的MTT分析显示出> 80％的活力。此外，PEC纤维增强水凝胶的共聚焦成像显示出明显的生存能力和增殖能力。使用FTIR研究了胶凝剂，聚电解质和羟基磷灰石之间的分子相互作用。我们研究了PEC纤维与βGP，NaHCO3和HAp之间的界面结合。羟基磷灰石与聚合物自组装技术的结合提高了可注射水凝胶的效率，这有助于微创应用。

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