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首页> 外文期刊>Materials science & engineering >The biocompatibility of silver and nanohydroxyapatite coatings on titanium dental implants with human primary osteoblast cells
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The biocompatibility of silver and nanohydroxyapatite coatings on titanium dental implants with human primary osteoblast cells

机译：银和纳米羟基磷灰石涂层在钛牙种植体上与人原代成骨细胞的生物相容性

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摘要

Silver nanoparticles (Ag NPs) are antimicrobial, with potential uses in medical implants, but Ag NPs alone can also be toxic to mammalian cells. This study aimed to enhance the biocompatibility of Ag NP-coated titanium dental implants with hydroxyapatite (HA) applied to the surface. Ti6Al4V discs were coated with Ag NPs, Ag NPs plus HA nanoparticles (Ag + nHA), or Ag NPs plus HA microparticles (Ag + mHA). The stability of coatings was explored and the biocompatibility with primary human osteoblasts over 7 days. Results showed that Ti6Al4V discs were successfully coated with silver and HA. The primary particle size of nHA and mHA were 23.90 +/- 1.49 nm and 4.72 +/- 0.38 mu m respectively. Metal analysis showed that underlying silver coatings remain stable in DMEM culture media, but the presence of FBS in the media caused some initial (clinically beneficial) release of dissolved silver. With additions of HA, osteoblasts were adherent, had normal morphology, negligible lactate dehydrogenase (LDH) leak, and showed alkaline phosphatase (ALP) activity. Cell viability was around 70% throughout the Ag + nHA treatment. Overall, the implants coated with Ag + nHA maintained a higher degree of biocompatibility compared to those coated with Ag + mHA, or Ag NPs alone, suggesting the former has a benefit for clinical use.
机译：银纳米颗粒（Ag NPs）具有抗菌作用，在医疗植入物中具有潜在用途，但单独的Ag NPs也可能对哺乳动物细胞有毒。这项研究旨在通过将羟基磷灰石（HA）应用于表面来增强Ag NP涂层的钛牙种植体的生物相容性。 Ti6Al4V圆盘涂有Ag NP，Ag NP加HA纳米颗粒（Ag + nHA）或Ag NPs加HA微粒（Ag + mHA）。探索了涂层的稳定性，并探讨了其与人类原代成骨细胞在7天内的生物相容性。结果表明，Ti6Al4V光盘已成功镀银和HA。 nHA和mHA的初级粒径分别为23.90 +/- 1.49nm和4.72 +/-0.38μm。金属分析表明，底层的银涂层在DMEM培养基中保持稳定，但是培养基中FBS的存在导致溶解的银开始一些初始释放（临床上有益）。加入HA后，成骨细胞粘附，形态正常，乳酸脱氢酶（LDH）泄漏可忽略不计，并显示碱性磷酸酶（ALP）活性。在整个Ag + nHA处理过程中，细胞活力约为70％。总体而言，与单独涂有Ag + mHA或单独使用Ag NPs的植入物相比，涂有Ag + nHA的植入物保持了更高的生物相容性，这表明前者对临床具有益处。

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  • 来源
    《Materials science & engineering》 |2020年第2期|110210.1-110210.11|共11页
  • 作者

    Salaie Ranj Nadhim; Besinis Alexandros; Le Huirong; Tredwin Christopher; Handy Richard D.;

  • 作者单位

    Univ Plymouth Fac Sci & Engn Sch Biol & Marine Sci Plymouth Devon England|Univ Plymouth Plymouth Univ Peninsula Sch Med & Dent Plymouth Devon England;

    Univ Plymouth Fac Sci & Engn Sch Biol & Marine Sci Plymouth Devon England|Univ Plymouth Fac Sci & Engn Sch Engn Plymouth Devon England|Univ Plymouth Plymouth Univ Peninsula Sch Med & Dent Plymouth Devon England;

    Univ Derby Coll Engn & Technol Sch Mech Engn & Built Environm Derby England;

    Univ Plymouth Plymouth Univ Peninsula Sch Med & Dent Plymouth Devon England;

    Univ Plymouth Fac Sci & Engn Sch Biol & Marine Sci Plymouth Devon England|Cihan Univ Erbil Dept Nutr Erbil Kurdistan Regio Iraq;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Implant coatings; Clinical safety; Metal toxicity; Dissolution; Amalar blue assay;

    机译：植入物涂料;临床安全;金属毒性;溶解;阿马拉蓝测定;

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