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首页> 外文期刊>Materials science & engineering >Polycaprolactone/gelatin-based scaffolds with tailored performance: in vitro and in vivo validation
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Polycaprolactone/gelatin-based scaffolds with tailored performance: in vitro and in vivo validation

机译：具有定制性能的聚己内酯/明胶基支架：体外和体内验证

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Nanofibrous scaffolds composed of polycaprolactone (PCL) and gelatin (Ge) were obtained through a hydrolytic assisted electrospinning process. The PCL-to-Ge proportion (100/0 to 20/80), as well as the dissolution time (24, 48, 72, 96, 120h) into a 1:1 formic/acetic acid solvent before electrospinning were modified to obtain the different samples. A strong influence of these factors on the physicochemical properties of the scaffolds was observed. Higher Ge percentage reduced crystallinity, allowed a uniform morphology and increased water contact angle. The increase in the dissolution time considerably reduced the molar mass and, subsequently, fibre diameter and crystallinity were affected. During in vitro biocompatibility tests, higher cell adhesion and proliferation were found for the 60/40, 50/50 and 40/60 PCL/Ge compositions that was corroborated by MTT assay, fluorescence and microscopy. A weakened structure, more labile to the in vitro degradation in physiologic conditions was found for these compositions with higher dissolution times (72 and 96 h). Particularly, the 40/60 PCL/Ge scaffolds revealed an interesting progressive degradation behaviour as a function of the dissolution time. Moreover, these scaffolds were non-inflammatory, as revealed by the pyrogen test and after the 15-day subcutaneous in vivo implantation in mice. Finally, a reduction of the scar tissue area after infarction was found for the 40/60 PCL/Ge scaffolds electrospun after 72 h implanted in rat hearts. These results are especially interesting and represent a feasible way to avoid undesired inflammatory reactions during the scaffold assimilation.
机译：通过水解辅助电纺丝工艺获得了由聚己内酯（PCL）和明胶（Ge）组成的纳米纤维支架。修改静电纺丝前PCL与Ge的比例（100/0至20/80）以及在1：1甲酸/乙酸溶剂中的溶解时间（24、48、72、96、120h）不同的样本。观察到这些因素对支架的理化性质的强烈影响。较高的Ge百分比降低了结晶度，允许均匀的形态并增加了水接触角。溶解时间的增加大大降低了摩尔质量，随后影响了纤维直径和结晶度。在体外生物相容性测试过程中，发现60 / 40、50 / 50和40/60 PCL / Ge组合物具有更高的细胞粘附性和增殖能力，这通过MTT分析，荧光和显微镜检查得到了证实。对于这些具有较高溶解时间（72和96小时）的组合物，发现了在生理条件下对体外降解更不稳定的弱化结构。特别地，40/60 PCL / Ge支架显示出有趣的渐进降解行为，它是溶解时间的函数。而且，如热原测试以及在小鼠中皮下体内植入15天后，这些支架是非炎性的。最后，发现在大鼠心脏中植入72小时后，电纺40/60 PCL / Ge支架在梗塞后疤痕组织面积减少。这些结果特别令人感兴趣，并且代表了避免支架吸收过程中不希望的炎症反应的可行方法。

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