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首页> 外文期刊>Materials science & engineering >Motor and sensitive recovery after injection of a physically cross-linked PNIPAAm-g-PEG hydrogel in rat hemisectioned spinal cord
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Motor and sensitive recovery after injection of a physically cross-linked PNIPAAm-g-PEG hydrogel in rat hemisectioned spinal cord

机译：在大鼠半切脊髓注射物理交联的PNIPAAm-g-PEG水凝胶后，运动和敏感恢复

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摘要

In line with experiments showing that implanted hydrogels are promising tools, we designed and injected, after a C2 spinal cord hemisection, a thermoresponsive and thermoreversible physically cross-linked poly(N-isopropylacrylamide)-poly(ethylene glycol) copolymer in order to reduce functional deficits and provide a favorable environment to axotomized axons.Nasal olfactory ecto-mesenchymal stern cells were cultured on the hydrogel in order to verify its biocompatibility. Then, inflammatory reaction (Interleukin-1 beta. and 6, Tumor Necrosis Factor-alpha) was examined 15 days post-hydrogel injection. Functional recovery (postural and locomotor activities, muscle strength and tactile sensitivity) was assessed once a week, during 12 weeks. Finally, at 12 weeks post-injection, spinal reflexivity and ventilatory adjustments were measured, and the presence of glial cells and regenerated axons were determined in the injured area.Our results indicate that cells survived and proliferated on the hydrogel which, itself, did not induce an enhanced inflammation. Furthermore, we observed significant motor and sensitive improvements in hydrogel-injected animals. Hydrogel also induced H-reflex recovery close to control animals but no improved ventilatory adjustment to electrically-evoked isometric contractions. Finally, regrowing axons were visualized within the hydrogel with no glial cells colonization.Our results emphasize the effectiveness of our copolymer and its high therapeutic potential to repair the spinal cord after injury.
机译：根据表明植入的水凝胶是有前途的工具的实验，我们在C2脊髓半切后设计并注射了一种热响应性和热可逆性物理交联的聚（N-异丙基丙烯酰胺）-聚（乙二醇）共聚物，以降低功能性。在水凝胶上培养鼻嗅外-间充质干细胞，以验证其生物相容性。然后，在水凝胶注射后15天检查炎症反应（白细胞介素1β和6，肿瘤坏死因子-α）。在12周内每周评估一次功能恢复（姿势和运动活动，肌肉力量和触觉敏感性）。最后，在注射后12周，测量了脊髓的反射性和通气调节，并确定了受损区域中神经胶质细胞和再生轴突的存在。我们的结果表明，细胞在水凝胶上存活和增殖，而水凝胶本身并没有引起炎症增强。此外，我们在注射水凝胶的动物中观察到了明显的运动和敏感性改善。水凝胶还诱导了接近对照动物的H反射恢复，但对电诱发的等距收缩的通气调节没有改善。最后，在水凝胶中可见到再生的轴突，没有胶质细胞定居。我们的结果强调了我们共聚物的有效性及其在损伤后修复脊髓的高治疗潜力。

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