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Bioreduction mechanism of silver nanoparticles

机译：银纳米颗粒的生物还原机理

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The studies represent in-depth investigations of bacteria mediated reduction mechanism of silver nanoparticles (Ag NPs) not reported so far. Ag NPs were successfully synthesized by Bacillus subtilis spizizenni (NCIM 2063), and their bioreduction mechanism has been explored in detail. Optical absorption spectrum shows surface plasmon resonance peak in region 408-423 nm. FT-IR analysis of Ag NPs showed a peak at 3435 cm(-1) which indicates the presence of N-H group (primary, secondary amines, and amides) on the surface of Ag NPs. XRD revealed the formation of a face-centered cubic crystal and hexagonal structure. TEM analysis indicates that bacterial, and media components affect size of nanoparticles. The detailed analysis of bioreduction mechanism was achieved using Sephadex G-25 column chromatography, HR-LCMS techniques, and nitrate reductase assay. The detection of capping agents was done by HR-MS. Result confirms the presence of nitrate reductase as well as other enzymes from bacterial supernatant and tripeptides from nutrient media involved in the reduction of silver. Furthermore, the confirmation of the origin of the tripeptides was investigated using BLAST technique. Three tripeptides (TYK, PFF, and YIF) from bacterial supernatant and one tripeptide (RWE) from nutrient media were detected on the surface of Ag NPs. Antibacterial efficiency of synthesized Ag NPs was evaluated against human pathogenic bacterial strains. Compared with other physical and chemical methods, the present method is cost-effective and environmentally benign using non-pathogenic bacteria.
机译：这些研究代表了迄今为止尚未报道的细菌介导的银纳米颗粒（Ag NPs）还原机制的深入研究。枯草芽孢杆菌（NCIM 2063）成功合成了银纳米颗粒，并详细探讨了其生物还原机理。光吸收光谱显示在408-423nm区域中的表面等离子体激元共振峰。 Ag NP的FT-IR分析显示在3435 cm（-1）处有一个峰，表明在Ag NP的表面上存在N-H基团（伯，仲胺和酰胺）。 XRD揭示了以面心立方晶体和六边形结构的形成。 TEM分析表明细菌和培养基成分会影响纳米粒子的尺寸。使用Sephadex G-25柱色谱，HR-LCMS技术和硝酸还原酶测定可对生物还原机理进行详细分析。封端剂的检测是通过HR-MS完成的。结果证实存在硝酸还原酶以及细菌上清液中的其他酶和来自涉及银还原的营养培养基的三肽。此外，使用BLAST技术研究了三肽的来源。在Ag NP的表面检测到了来自细菌上清液的三个三肽（TYK，PFF和YIF）和来自营养培养基的三个三肽（RWE）。评估了合成的Ag NPs对人类致病细菌菌株的抗菌效率。与其他物理和化学方法相比，本方法使用非致病性细菌具有成本效益并且对环境无害。

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