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首页> 外文期刊>Materials science & engineering >Response of human periosteal cells to degradation products of zinc and its alloy
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Response of human periosteal cells to degradation products of zinc and its alloy

机译：人骨膜细胞对锌及其合金降解产物的反应

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摘要

Zinc (Zn) and its alloys are proposed as promising resorbable materials for osteosynthesis implants. Detailed studies should be undertaken to clarify their properties in terms of degradability, biocompatibility and osteoinductivity. Degradation products of Zn alloys might affect directly adjacent cellular and tissue responses. Periosteal stem cells are responsible for participating in intramembranous ossification during fracture healing. The present study aims at examining possible effects emanating from Zn or Zn-4Ag (wt%) alloy degradation products on cell viability and osteogenic differentiation of a human immortalized cranial periosteal cell line (TAg cells). Therefore, a modified extraction method was used to investigate the degradation behavior of Zn and Zn-4Ag alloys under cell culture conditions. Compared with pure Zn, Zn-4Ag alloy showed almost fourfold higher degradation rates under cell culture conditions, while the associated degradation products had no adverse effects on cell viability. Osteogenic induction of TAg cells revealed that high concentration extracts significantly reduced calcium deposition of TAg cells, while low concentration extracts enhanced calcium deposition, indicating a dose-dependent effect of Zn ions. Our results give evidence that the observed cytotoxicity effects were determined by the released degradation products of Zn and Zn-4Ag alloys, rather than by degradation rates calculated by weight loss. Extracellular Zn ion concentration was found to modulate osteogenic differentiation of TAg cells. These findings provide significant implications and guidance for the development of Zn-based alloys with an optimized degradation behavior for Zn-based osteosynthesis implants.
机译：提出了锌（Zn）及其合金作为用于骨合成植入物的有前途的可吸收材料。应进行详细研究，以从降解性，生物相容性和骨诱导性方面阐明其性质。锌合金的降解产物可能会直接影响邻近细胞和组织的反应。骨膜干细胞负责骨折愈合过程中的膜内骨化。本研究旨在研究锌或Zn-4Ag（wt％）合金降解产物对人永生化颅骨骨膜细胞系（TAg细胞）细胞活力和成骨分化的可能影响。因此，采用改良的提取方法研究了Zn和Zn-4Ag合金在细胞培养条件下的降解行为。与纯锌相比，Zn-4Ag合金在细胞培养条件下的降解率高出近四倍，而相关的降解产物对细胞活力没有不利影响。 TAg细胞的成骨诱导显示高浓度提取物显着降低了TAg细胞的钙沉积，而低浓度提取物则增强了钙沉积，表明Zn离子具有剂量依赖性。我们的结果提供了证据，表明观察到的细胞毒性作用是由Zn和Zn-4Ag合金释放的降解产物决定的，而不是由重量损失计算的降解率决定的。发现细胞外Zn离子浓度调节TAg细胞的成骨分化。这些发现为开发具有最佳降解行为的锌基骨合成植入物的锌基合金提供了重要的启示和指导。

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  • 来源
    《Materials science & engineering》 |2020年第3期|110208.1-110208.11|共11页
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  • 作者单位

    Univ Hosp Tubingen Sect Med Mat Sci & Technol Osianderstr 2-8 D-72074 Tubingen Germany;

    Univ Hosp Tubingen Dept Oral & Maxillofacial Surg Osianderstr 2-8 D-72076 Tubingen Germany;

    Schwabisch Gmund Res Inst Precious Met & Met Chem Fem Katharinenstr 17 D-73525 Schwabisch Gmund Germany;

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  • 正文语种 eng
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  • 关键词

    Absorbable metals; Zinc alloy; Degradation; Cytocompatibility; Osteogenesis; Periosteal cells;

    机译：可吸收金属;锌合金降解;细胞相容性;成骨;骨膜细胞;

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