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首页> 外文期刊>Materials science & engineering >AgNPs/QDs@GQDs nanocomposites developed as an ultrasensitive impedimetric aptasensor for ractopamine detection
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AgNPs/QDs@GQDs nanocomposites developed as an ultrasensitive impedimetric aptasensor for ractopamine detection

机译：AgNPs / QDs @ GQDs纳米复合材料开发为用于莱克多巴胺检测的超灵敏阻抗适体传感器

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Developing easy-to-use and miniaturized sensors for in-field monitoring of targets which is related to human health is necessary. Ractopamine (RAC) is a feed additive with serious side effects that is forbidden in many countries. This study reports the fabrication of an impedimetric aptasensor for ultrasensitive and selective detection of the RAC in human biological fluids. Accordingly, an efficient nanocomposites was synthesized by a beneficial combination of graphene quantum dots (GQDs), quantum dots (QDs) and silver nanoparticles (AgNPs) for modifying a glassy carbon electrode (GCE). This nanocomposite is promising to present a synergistic effect in the increase of the active surface area of the modified electrode to more load the biocapture of the target. Next, the RAC-binding aptamer (Apt) was attached to the AgNPs/QDs@GQDs/GCE surface and a sensitive layer for the RAC detection was embedded. A RAC-Apt complex was formed upon adding the RAC and the changes of the electrochemical behavior were studied by some electrochemical techniques such as electrochemical impedance spectroscopy (EIS). Under optimal conditions, the charge transfer resistance (R-ct) value was increased linearly with increasing of the RAC concentrations in the range of 1 fM to 901.4 nM. Limit of detection (LOD) was calculated to be 330 aM which is superior by other reported electrochemical methods in the RAC sensing. The applicability of the aptasensor was tested in human urine and blood serum as the real samples and satisfactory results of specificity were achieved. It seems that the proposed strategy not only provides a new ultrasensitive strategy for RAC detection but also expands the application of the sensing interface to develop other aptasensors by changing the Apt sequence.
机译：必须开发易于使用且小型化的传感器，以对与人类健康有关的目标进行现场监控。莱克多巴胺（RAC）是一种饲料添加剂，具有许多国家禁止的严重副作用。这项研究报告了用于人体生物流体中RAC的超灵敏和选择性检测的阻抗式适体传感器的制造。因此，通过石墨烯量子点（GQD），量子点（QD）和银纳米颗粒（AgNPs）的有益组合合成了有效的纳米复合材料，用于修饰玻璃碳电极（GCE）。该纳米复合材料有望在修饰电极的活性表面积增加中产生协同效应，从而更多地负载靶的生物捕获物。接下来，将RAC结合适体（Apt）附着到AgNPs / QDs @ GQDs / GCE表面，并嵌入用于RAC检测的敏感层。加入RAC后形成了RAC-Apt配合物，并通过电化学阻抗谱（EIS）等电化学技术研究了电化学行为的变化。在最佳条件下，电荷转移电阻（R-ct）值随着RAC浓度的增加在1 fM至901.4 nM范围内线性增加。计算的检出限（LOD）为330 aM，在RAC感测中通过其他报道的电化学方法优于。在人体尿液和血清中检测了适体传感器作为真实样品的适用性，并获得了令人满意的特异性结果。看来，提出的策略不仅为RAC检测提供了一种新的超灵敏策略，而且还通过改变Apt序列扩展了传感接口的应用，以开发其他适体传感器。

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